BDNF gene can be activated by Ca2+ signals without involvement of de novo AP-1 synthesis

Kuniaki Sano, Hikaru Nanba, Akiko Tabuchi, Tomofusa Tsuchiya, Masaaki Tsuda*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

44 被引用数 (Scopus)

抄録

Although stimulation of N-methyl-D-aspartate receptors or voltage-dependent calcium channels induces both the activation of c-fos and brain-derived neurotrophic factor (BDNF) genes, it is not certain how the activation of these genes is related. Using primary cultures of rat hippocampal neurons, we found that exposing the cells to cycloheximide allowed subsequent activation of BDNF mRNA expression, although activation of AP-1 DNA-binding activity resulting from the c-fos induction was abolished. Super-induction of BDNF gene was also caused by cycloheximide. The estimated half-life of BDNF mRNA was approximately 2.5 hrs, which was almost identical to that of c-fos mRNA. These results indicate that nascent AP-1 is not required for the activation of BDNF gene, leading to the notion that the BDNF gene can be activated by Ca2+ signals as an immediate early gene.

本文言語英語
ページ(範囲)788-793
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
229
3
DOI
出版ステータス出版済み - 1996/12/24

ASJC Scopus 主題領域

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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