Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA

Eiji Kobayashi; Sotaro Motoi; Masahito Sugiura; Masunori Kajikawa; Shuji Kojima; Junya Kohroki; Yasuhiko Masuho

doi:10.1016/j.imlet.2014.05.004

Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA

Eiji Kobayashi^*, Sotaro Motoi, Masahito Sugiura, Masunori Kajikawa, Shuji Kojima, Junya Kohroki, Yasuhiko Masuho

^*この論文の責任著者

免疫学講座

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

6 被引用数 (Scopus)

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Antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells is a major mechanism of tumor therapy with antibodies. NK cells not only manifest cytotoxicity but also secrete a variety of cytokines/chemokines that regulate immune responses. Using a retroviral vector, in this study we established a KHYG-1 cell line that stably expresses FcγRIIIA (CD16A). The KHYG-1/FcγRIIIA cells exerted potent antibody concentration-dependent ADCC, whereas parental KHYG-1 cells did not. In contrast, without antibody, the natural killer activity of KHYG-1/FcγRIIIA cells was less potent than that of parental KHYG-1 cells. During the course of ADCC, KHYG-1/FcγRIIIA cells secreted IFN-γ and MIP-1α dependent upon antibody concentration, but parental KHYG-1 cells did not. These results suggest that KHYG-1/FcγRIIIA cells would be useful in studies to elucidate the function of NK cells and the mechanism of ADCC.

本文言語	英語
ページ（範囲）	59-64
ページ数	6
ジャーナル	Immunology Letters
巻	161
号	1
DOI	https://doi.org/10.1016/j.imlet.2014.05.004
出版ステータス	出版済み - 2014/09

ASJC Scopus 主題領域

免疫アレルギー学
免疫学

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10.1016/j.imlet.2014.05.004

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@article{0ddc6ffeb47645b8b4dd54b4e185100e,

title = "Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA",

abstract = "Antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells is a major mechanism of tumor therapy with antibodies. NK cells not only manifest cytotoxicity but also secrete a variety of cytokines/chemokines that regulate immune responses. Using a retroviral vector, in this study we established a KHYG-1 cell line that stably expresses FcγRIIIA (CD16A). The KHYG-1/FcγRIIIA cells exerted potent antibody concentration-dependent ADCC, whereas parental KHYG-1 cells did not. In contrast, without antibody, the natural killer activity of KHYG-1/FcγRIIIA cells was less potent than that of parental KHYG-1 cells. During the course of ADCC, KHYG-1/FcγRIIIA cells secreted IFN-γ and MIP-1α dependent upon antibody concentration, but parental KHYG-1 cells did not. These results suggest that KHYG-1/FcγRIIIA cells would be useful in studies to elucidate the function of NK cells and the mechanism of ADCC.",

keywords = "ADCC, CD16A, FcγRIIIA, KHYG-1 cells, NK cells",

author = "Eiji Kobayashi and Sotaro Motoi and Masahito Sugiura and Masunori Kajikawa and Shuji Kojima and Junya Kohroki and Yasuhiko Masuho",

note = "Funding Information: This study was supported by Tokyo University of Science and carried out as a part of “The Project for New Functional Antibody Technologies” of the New Energy and Industrial Technology Development Organization (NEDO).",

year = "2014",

month = sep,

doi = "10.1016/j.imlet.2014.05.004",

language = "英語",

volume = "161",

pages = "59--64",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA

AU - Kobayashi, Eiji

AU - Motoi, Sotaro

AU - Sugiura, Masahito

AU - Kajikawa, Masunori

AU - Kojima, Shuji

AU - Kohroki, Junya

AU - Masuho, Yasuhiko

N1 - Funding Information: This study was supported by Tokyo University of Science and carried out as a part of “The Project for New Functional Antibody Technologies” of the New Energy and Industrial Technology Development Organization (NEDO).

PY - 2014/9

Y1 - 2014/9

N2 - Antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells is a major mechanism of tumor therapy with antibodies. NK cells not only manifest cytotoxicity but also secrete a variety of cytokines/chemokines that regulate immune responses. Using a retroviral vector, in this study we established a KHYG-1 cell line that stably expresses FcγRIIIA (CD16A). The KHYG-1/FcγRIIIA cells exerted potent antibody concentration-dependent ADCC, whereas parental KHYG-1 cells did not. In contrast, without antibody, the natural killer activity of KHYG-1/FcγRIIIA cells was less potent than that of parental KHYG-1 cells. During the course of ADCC, KHYG-1/FcγRIIIA cells secreted IFN-γ and MIP-1α dependent upon antibody concentration, but parental KHYG-1 cells did not. These results suggest that KHYG-1/FcγRIIIA cells would be useful in studies to elucidate the function of NK cells and the mechanism of ADCC.

AB - Antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells is a major mechanism of tumor therapy with antibodies. NK cells not only manifest cytotoxicity but also secrete a variety of cytokines/chemokines that regulate immune responses. Using a retroviral vector, in this study we established a KHYG-1 cell line that stably expresses FcγRIIIA (CD16A). The KHYG-1/FcγRIIIA cells exerted potent antibody concentration-dependent ADCC, whereas parental KHYG-1 cells did not. In contrast, without antibody, the natural killer activity of KHYG-1/FcγRIIIA cells was less potent than that of parental KHYG-1 cells. During the course of ADCC, KHYG-1/FcγRIIIA cells secreted IFN-γ and MIP-1α dependent upon antibody concentration, but parental KHYG-1 cells did not. These results suggest that KHYG-1/FcγRIIIA cells would be useful in studies to elucidate the function of NK cells and the mechanism of ADCC.

KW - ADCC

KW - CD16A

KW - FcγRIIIA

KW - KHYG-1 cells

KW - NK cells

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U2 - 10.1016/j.imlet.2014.05.004

DO - 10.1016/j.imlet.2014.05.004

M3 - 学術論文

C2 - 24841426

AN - SCOPUS:84901610096

SN - 0165-2478

VL - 161

SP - 59

EP - 64

JO - Immunology Letters

JF - Immunology Letters

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ER -

Antibody-dependent cellular cytotoxicity and cytokine/chemokine secretion by KHYG-1 cells stably expressing FcγRIIIA

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