An isotope-coded fluorogenic cross-linker for high-performance target identification based on photoaffinity labeling

Takenori Tomohiro*, Shota Morimoto, Toshiya Shima, Junya Chiba, Yasumaru Hatanaka

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)

抄録

A photoaffinity labeling (PAL)-based method for the rapid identification of target proteins is presented in which a high-performance chemical tag, an isotope-coded fluorescent tag (IsoFT), can be attached to the interacting site by irradiation. Labeled peptides can be easily distinguished among numerous proteolytic digests by sequential detection with highly sensitive fluorescence spectroscopy and mass spectrometry. Subsequent MS/MS analysis provides amino acid sequence information with a higher depth of coverage. The combination of PAL and heterogeneous target-selecting techniques significantly reduces the amount of time and protein required for identification. An additional photocleavable moiety successfully accelerated proteomic analysis using cell lysate. This method is a widely applicable approach for the rapid and accurate identification of interacting proteins.

本文言語英語
ページ(範囲)13502-13505
ページ数4
ジャーナルAngewandte Chemie - International Edition
53
49
DOI
出版ステータス出版済み - 2014/11/07

ASJC Scopus 主題領域

  • 触媒
  • 化学一般

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