抄録
Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3′-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5′-proximal sgRNA genes in vitro.
本文言語 | 英語 |
---|---|
ページ(範囲) | 2591-2597 |
ページ数 | 7 |
ジャーナル | FEBS Letters |
巻 | 580 |
号 | 11 |
DOI | |
出版ステータス | 出版済み - 2006/05/15 |
ASJC Scopus 主題領域
- 生物理学
- 構造生物学
- 生化学
- 分子生物学
- 遺伝学
- 細胞生物学