A translational enhancer element on the 3′-proximal end of the Panicum mosaic virus genome

Jeffrey S. Batten; Benedicte Desvoyes; Yoshimi Yamamura; Karen Beth G. Scholthof

doi:10.1016/j.febslet.2006.04.006

A translational enhancer element on the 3′-proximal end of the Panicum mosaic virus genome

Jeffrey S. Batten, Benedicte Desvoyes, Yoshimi Yamamura, Karen Beth G. Scholthof^*

^*Corresponding author for this work

Plant Resource Sciences

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3′-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5′-proximal sgRNA genes in vitro.

Original language	English
Pages (from-to)	2591-2597
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2006.04.006
State	Published - 2006/05/15

Keywords

3-untranslated region
Cap-independent translation
Panicovirus
RNA structure
Translation strategies

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.04.006

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@article{3ce73881449549f0b196154ca95ec8d5,

title = "A translational enhancer element on the 3′-proximal end of the Panicum mosaic virus genome",

abstract = "Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3′-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5′-proximal sgRNA genes in vitro.",

keywords = "3-untranslated region, Cap-independent translation, Panicovirus, RNA structure, Translation strategies",

author = "Batten, {Jeffrey S.} and Benedicte Desvoyes and Yoshimi Yamamura and Scholthof, {Karen Beth G.}",

note = "Funding Information: This work was funded by USDA-NRI (99-3503-7974) and THECB-ATP (000517-0043-2003) grants awarded to K.-B.G. Scholthof. We thank Herman Scholthof, Rustem Omarov, and the two anonymous referees for their helpful suggestions and criticism of this manuscript. ",

year = "2006",

month = may,

day = "15",

doi = "10.1016/j.febslet.2006.04.006",

language = "英語",

volume = "580",

pages = "2591--2597",

journal = "FEBS Letters",

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T1 - A translational enhancer element on the 3′-proximal end of the Panicum mosaic virus genome

AU - Batten, Jeffrey S.

AU - Desvoyes, Benedicte

AU - Yamamura, Yoshimi

AU - Scholthof, Karen Beth G.

N1 - Funding Information: This work was funded by USDA-NRI (99-3503-7974) and THECB-ATP (000517-0043-2003) grants awarded to K.-B.G. Scholthof. We thank Herman Scholthof, Rustem Omarov, and the two anonymous referees for their helpful suggestions and criticism of this manuscript.

PY - 2006/5/15

Y1 - 2006/5/15

N2 - Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3′-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5′-proximal sgRNA genes in vitro.

AB - Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3′-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5′-proximal sgRNA genes in vitro.

KW - 3-untranslated region

KW - Cap-independent translation

KW - Panicovirus

KW - RNA structure

KW - Translation strategies

UR - http://www.scopus.com/inward/record.url?scp=33745569385&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.04.006

DO - 10.1016/j.febslet.2006.04.006

M3 - 学術論文

C2 - 16647707

AN - SCOPUS:33745569385

SN - 0014-5793

VL - 580

SP - 2591

EP - 2597

JO - FEBS Letters

JF - FEBS Letters

IS - 11

ER -

A translational enhancer element on the 3′-proximal end of the Panicum mosaic virus genome

Abstract

Keywords

ASJC Scopus subject areas

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