A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Jong Min Kim; Masayasu Suzuki; Rolf D. Schmid

doi:10.1080/00032718908052365

A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Jong Min Kim^*, Masayasu Suzuki, Rolf D. Schmid

^*この論文の責任著者

工学科　電気電子工学コース

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

5 被引用数 (Scopus)

抄録

Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

本文言語	英語
ページ（範囲）	2433-2443
ページ数	11
ジャーナル	Analytical Letters
巻	22
号	11-12
DOI	https://doi.org/10.1080/00032718908052365
出版ステータス	出版済み - 1989/01

ASJC Scopus 主題領域

分析化学
生化学
分光学
臨床生化学
生化学、医学
電気化学

文献へのアクセス

10.1080/00032718908052365

引用スタイル

@article{8a1a2b4f877f4a558fcff9d69b3bfeda,

title = "A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode",

abstract = "Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.",

keywords = "Hypoxanthine, oxygen electrode, sulfite, xanthine, xanthine oxidase assay",

author = "Kim, {Jong Min} and Masayasu Suzuki and Schmid, {Rolf D.}",

year = "1989",

month = jan,

doi = "10.1080/00032718908052365",

language = "英語",

volume = "22",

pages = "2433--2443",

journal = "Analytical Letters",

issn = "0003-2719",

publisher = "Taylor and Francis Ltd.",

number = "11-12",

}

TY - JOUR

T1 - A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

AU - Kim, Jong Min

AU - Suzuki, Masayasu

AU - Schmid, Rolf D.

PY - 1989/1

Y1 - 1989/1

N2 - Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

AB - Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

KW - Hypoxanthine

KW - oxygen electrode

KW - sulfite

KW - xanthine

KW - xanthine oxidase assay

UR - http://www.scopus.com/inward/record.url?scp=0024806017&partnerID=8YFLogxK

U2 - 10.1080/00032718908052365

DO - 10.1080/00032718908052365

M3 - 学術論文

AN - SCOPUS:0024806017

SN - 0003-2719

VL - 22

SP - 2433

EP - 2443

JO - Analytical Letters

JF - Analytical Letters

IS - 11-12

ER -

A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

抄録

ASJC Scopus 主題領域

文献へのアクセス

フィンガープリント

引用スタイル