A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Jong Min Kim; Masayasu Suzuki; Rolf D. Schmid

doi:10.1080/00032718908052365

A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Jong Min Kim^*, Masayasu Suzuki, Rolf D. Schmid

^*Corresponding author for this work

Electric and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

Original language	English
Pages (from-to)	2433-2443
Number of pages	11
Journal	Analytical Letters
Volume	22
Issue number	11-12
DOIs	https://doi.org/10.1080/00032718908052365
State	Published - 1989/01

Keywords

Hypoxanthine
oxygen electrode
sulfite
xanthine
xanthine oxidase assay

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032718908052365

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title = "A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode",

abstract = "Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.",

keywords = "Hypoxanthine, oxygen electrode, sulfite, xanthine, xanthine oxidase assay",

author = "Kim, {Jong Min} and Masayasu Suzuki and Schmid, {Rolf D.}",

year = "1989",

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doi = "10.1080/00032718908052365",

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journal = "Analytical Letters",

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T1 - A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

AU - Kim, Jong Min

AU - Suzuki, Masayasu

AU - Schmid, Rolf D.

PY - 1989/1

Y1 - 1989/1

N2 - Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

AB - Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.

KW - Hypoxanthine

KW - oxygen electrode

KW - sulfite

KW - xanthine

KW - xanthine oxidase assay

UR - http://www.scopus.com/inward/record.url?scp=0024806017&partnerID=8YFLogxK

U2 - 10.1080/00032718908052365

DO - 10.1080/00032718908052365

M3 - 学術論文

AN - SCOPUS:0024806017

SN - 0003-2719

VL - 22

SP - 2433

EP - 2443

JO - Analytical Letters

JF - Analytical Letters

IS - 11-12

ER -

A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode

Abstract

Keywords

ASJC Scopus subject areas

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