TY - JOUR
T1 - Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells
AU - Lirdprapamongkol, Kriengsak
AU - Sakurai, Hiroaki
AU - Kawasaki, Noritaka
AU - Choo, Min Kyung
AU - Saitoh, Yurika
AU - Aozuka, Yasushi
AU - Singhirunnusorn, Pattama
AU - Ruchirawat, Somsak
AU - Svasti, Jisnuson
AU - Saiki, Ikuo
N1 - Funding Information:
This work was supported by a Japanese-Thai Collaborative Scientific Research Fellowship (JSPS-NRCT), 2003. We would like to thank Dr. Montip Tiensuwan, Department of Mathematics, Faculty of Science, Mahidol University, for her assistance with the statistical analysis.
PY - 2005/5
Y1 - 2005/5
N2 - Vanillin, a food flavoring agent, has been reported to show anti-mutagenic activity and to inhibit chemical carcinogenesis. In this study, we examined the effect of vanillin on the growth and metastasis of 4T1 mammary adenocarcinoma cells in BALB/c mice. Mice orally administered with vanillin showed significantly reduced numbers of lung metastasized colonies compared to controls. In vitro studies revealed that vanillin, at concentrations that were not cytotoxic, inhibited invasion and migration of cancer cells and inhibited enzymatic activity of MMP-9 secreted by the cancer cells. Vanillin also showed growth inhibitory effect towards cancer cells in vitro. However, vanillic acid, a major metabolic product of vanillin in human and rat, was not active in these in vitro activity assays. Our findings suggest that vanillin has anti-metastatic potential by decreasing invasiveness of cancer cells. Since vanillin is generally regarded as safe, it may be of value in the development of anti-metastatic drugs for cancer treatment.
AB - Vanillin, a food flavoring agent, has been reported to show anti-mutagenic activity and to inhibit chemical carcinogenesis. In this study, we examined the effect of vanillin on the growth and metastasis of 4T1 mammary adenocarcinoma cells in BALB/c mice. Mice orally administered with vanillin showed significantly reduced numbers of lung metastasized colonies compared to controls. In vitro studies revealed that vanillin, at concentrations that were not cytotoxic, inhibited invasion and migration of cancer cells and inhibited enzymatic activity of MMP-9 secreted by the cancer cells. Vanillin also showed growth inhibitory effect towards cancer cells in vitro. However, vanillic acid, a major metabolic product of vanillin in human and rat, was not active in these in vitro activity assays. Our findings suggest that vanillin has anti-metastatic potential by decreasing invasiveness of cancer cells. Since vanillin is generally regarded as safe, it may be of value in the development of anti-metastatic drugs for cancer treatment.
KW - Anti-metastatic
KW - Breast cancer
KW - Matrix metalloproteinase
KW - Migration
UR - http://www.scopus.com/inward/record.url?scp=20944434886&partnerID=8YFLogxK
U2 - 10.1016/j.ejps.2005.01.015
DO - 10.1016/j.ejps.2005.01.015
M3 - 学術論文
C2 - 15854801
AN - SCOPUS:20944434886
SN - 0928-0987
VL - 25
SP - 57
EP - 65
JO - European Journal of Pharmaceutical Sciences
JF - European Journal of Pharmaceutical Sciences
IS - 1
ER -
