TY - JOUR
T1 - Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats
AU - Hikiami, Hiroaki
AU - Kimura, Mari
AU - Shibahara, Naotoshi
AU - Yoshida, Toshiko
AU - Jo, Michiko
AU - Kaneko, Maria
AU - Nogami, Tatsuya
AU - Fujimoto, Makoto
AU - Goto, Hirozo
AU - Shimada, Yutaka
PY - 2011
Y1 - 2011
N2 - We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased -smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.
AB - We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased -smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.
UR - http://www.scopus.com/inward/record.url?scp=79959206789&partnerID=8YFLogxK
U2 - 10.1155/2011/592791
DO - 10.1155/2011/592791
M3 - 学術論文
C2 - 21660308
AN - SCOPUS:79959206789
SN - 1741-427X
VL - 2011
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 592791
ER -