Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats

Hiroaki Hikiami*, Mari Kimura, Naotoshi Shibahara, Toshiko Yoshida, Michiko Jo, Maria Kaneko, Tatsuya Nogami, Makoto Fujimoto, Hirozo Goto, Yutaka Shimada

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased -smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

Original languageEnglish
Article number592791
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Complementary and alternative medicine

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