TY - JOUR
T1 - Synthesis of alkynyl C-nucleotide triphosphates toward enzymatic elongation of artificial DNA
AU - Oda, Yutaro
AU - Chiba, Junya
AU - Inouye, Masahiko
N1 - Publisher Copyright:
© 2018 The Japan Institute of Heterocyclic Chemistry
PY - 2018
Y1 - 2018
N2 - Four types of alkynyl C-nucleotide triphosphates were synthesized, in which heterocyclic compounds as a non-natural nucleobase were tethered to deoxyribose through acetylene bonds. The non-natural nucleobases are D*, T*, G*, and C*, corresponding to natural A, T, G, and C, respectively, from a hydrogen-bonding pattern of view. All the non-natural triphosphates were found to be a good substrate for terminal deoxynucleotidyl transferase (TdT), a natural nucleic acid-relevant enzyme.
AB - Four types of alkynyl C-nucleotide triphosphates were synthesized, in which heterocyclic compounds as a non-natural nucleobase were tethered to deoxyribose through acetylene bonds. The non-natural nucleobases are D*, T*, G*, and C*, corresponding to natural A, T, G, and C, respectively, from a hydrogen-bonding pattern of view. All the non-natural triphosphates were found to be a good substrate for terminal deoxynucleotidyl transferase (TdT), a natural nucleic acid-relevant enzyme.
UR - http://www.scopus.com/inward/record.url?scp=85068226378&partnerID=8YFLogxK
U2 - 10.3987/COM-18-S(T)48
DO - 10.3987/COM-18-S(T)48
M3 - 学術論文
AN - SCOPUS:85068226378
SN - 0385-5414
VL - 97
SP - 612
EP - 620
JO - Heterocycles
JF - Heterocycles
IS - 1
ER -