@article{b8336f16783d402b95dbe0a19f1f10b1,
title = "Survival of parvovirus B19-infected cells by cellular autophagy",
abstract = "Human parvovirus B19 (B19) is a well-known pathogenic agent which causes apoptosis in erythrocyte lineage cells. Here, we provide the first evidence that mitochondrial autophagy is specifically found in the B19-infected cells. The protein expression ratio for LC3-II/LC3-I increased significantly in infected cells, indicating possible involvement of cellular autophagy in the infection process. Immunofluorescence confocal microscopy analyses revealed that B19 infection induced an intracellular autophagosome as judged by endogenous LC3 staining. Moreover, inhibition of autophagy by 3-MA significantly facilitated B19-infection-mediated cell death. These results suggest a novel mechanism by which B19-infected cells survive by cellular autophagy.",
keywords = "3-MA, Autophagy, LC3, Parvovirus B19",
author = "Akitoshi Nakashima and Nobuyuki Tanaka and Keiichi Tamai and Masanao Kyuuma and Yoshinori Ishikawa and Hiroyuki Sato and Tamotsu Yoshimori and Shigeru Saito and Kazuo Sugamura",
note = "Funding Information: We gratefully acknowledge the gift of recombinant Epo from Kirin Brewery Pharmaceutical Research Laboratory, Tokyo, Japan. The authors thank Mr. Lamichhane Aayam for critically reading the manuscript. This work was supported in part by a Grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), the 21st century Center of Excellence (COE) Program and Grant-in-aids for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports, and Culture, a Grant-in-aid from the Ministry of Health, Labor, and Welfare of the Japanese Government. K.T. and M.K. are JSPS research fellows.",
year = "2006",
month = jun,
day = "5",
doi = "10.1016/j.virol.2006.03.029",
language = "英語",
volume = "349",
pages = "254--263",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",
}