Stimulation of cultured colon 26 cells with TNF-α promotes lung metastasis through the extracellular signal-regulated kinase pathway

Min Kyung Choo; Hiroaki Sakurai; Keiichi Koizumi; Ikuo Saiki

doi:10.1016/j.canlet.2004.12.027

Stimulation of cultured colon 26 cells with TNF-α promotes lung metastasis through the extracellular signal-regulated kinase pathway

Min Kyung Choo, Hiroaki Sakurai^*, Keiichi Koizumi, Ikuo Saiki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We investigated the influence of TNF-α on the metastasis of cancer cells. Treatment of cultured colon 26 cells with TNF-α enhanced metastatic properties including production of MMP-9, adhesion, migration and invasion. Cells treated with TNF-α in vitro showed marked potential to metastasize to the lung and liver in vivo. U0126, an inhibitor of MEK1/2, inhibited the TNF-α-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and the metastatic properties in vitro without affecting cell proliferation. In addition, pretreatment with U0126 in vitro completely abrogated the increased lung metastasis of TNF-α-treated cells. These results indicate that TNF-α-induced activation of cancer cells through the ERK pathway is sufficient for the enhanced metastatic potential of colon 26 cells.

Original language	English
Pages (from-to)	47-56
Number of pages	10
Journal	Cancer Letters
Volume	230
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2004.12.027
State	Published - 2005/12/08

Keywords

Extracellular signal-regulated kinase
Matrix metalloproteinase-9
Metastasis
Tumor necrosis factor-α

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.12.027

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@article{79027fdebc834bd9add75e2baeb67550,

title = "Stimulation of cultured colon 26 cells with TNF-α promotes lung metastasis through the extracellular signal-regulated kinase pathway",

abstract = "We investigated the influence of TNF-α on the metastasis of cancer cells. Treatment of cultured colon 26 cells with TNF-α enhanced metastatic properties including production of MMP-9, adhesion, migration and invasion. Cells treated with TNF-α in vitro showed marked potential to metastasize to the lung and liver in vivo. U0126, an inhibitor of MEK1/2, inhibited the TNF-α-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and the metastatic properties in vitro without affecting cell proliferation. In addition, pretreatment with U0126 in vitro completely abrogated the increased lung metastasis of TNF-α-treated cells. These results indicate that TNF-α-induced activation of cancer cells through the ERK pathway is sufficient for the enhanced metastatic potential of colon 26 cells.",

keywords = "Extracellular signal-regulated kinase, Matrix metalloproteinase-9, Metastasis, Tumor necrosis factor-α",

author = "Choo, {Min Kyung} and Hiroaki Sakurai and Keiichi Koizumi and Ikuo Saiki",

note = "Funding Information: This work was supported in part by Grant-in-Aids for Young Scientists (B) (No. 15790040), Cancer Research (No. 16022224 and 16023225) and 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and grants from the Uehara Memorial Foundation, Tokyo Biochemical Research Foundation, and First Bank of Toyama Foundation.",

year = "2005",

month = dec,

day = "8",

doi = "10.1016/j.canlet.2004.12.027",

language = "英語",

volume = "230",

pages = "47--56",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - Stimulation of cultured colon 26 cells with TNF-α promotes lung metastasis through the extracellular signal-regulated kinase pathway

AU - Choo, Min Kyung

AU - Sakurai, Hiroaki

AU - Koizumi, Keiichi

AU - Saiki, Ikuo

N1 - Funding Information: This work was supported in part by Grant-in-Aids for Young Scientists (B) (No. 15790040), Cancer Research (No. 16022224 and 16023225) and 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and grants from the Uehara Memorial Foundation, Tokyo Biochemical Research Foundation, and First Bank of Toyama Foundation.

PY - 2005/12/8

Y1 - 2005/12/8

N2 - We investigated the influence of TNF-α on the metastasis of cancer cells. Treatment of cultured colon 26 cells with TNF-α enhanced metastatic properties including production of MMP-9, adhesion, migration and invasion. Cells treated with TNF-α in vitro showed marked potential to metastasize to the lung and liver in vivo. U0126, an inhibitor of MEK1/2, inhibited the TNF-α-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and the metastatic properties in vitro without affecting cell proliferation. In addition, pretreatment with U0126 in vitro completely abrogated the increased lung metastasis of TNF-α-treated cells. These results indicate that TNF-α-induced activation of cancer cells through the ERK pathway is sufficient for the enhanced metastatic potential of colon 26 cells.

AB - We investigated the influence of TNF-α on the metastasis of cancer cells. Treatment of cultured colon 26 cells with TNF-α enhanced metastatic properties including production of MMP-9, adhesion, migration and invasion. Cells treated with TNF-α in vitro showed marked potential to metastasize to the lung and liver in vivo. U0126, an inhibitor of MEK1/2, inhibited the TNF-α-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and the metastatic properties in vitro without affecting cell proliferation. In addition, pretreatment with U0126 in vitro completely abrogated the increased lung metastasis of TNF-α-treated cells. These results indicate that TNF-α-induced activation of cancer cells through the ERK pathway is sufficient for the enhanced metastatic potential of colon 26 cells.

KW - Extracellular signal-regulated kinase

KW - Matrix metalloproteinase-9

KW - Metastasis

KW - Tumor necrosis factor-α

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U2 - 10.1016/j.canlet.2004.12.027

DO - 10.1016/j.canlet.2004.12.027

M3 - 学術論文

C2 - 16253760

AN - SCOPUS:19944412297

SN - 0304-3835

VL - 230

SP - 47

EP - 56

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Stimulation of cultured colon 26 cells with TNF-α promotes lung metastasis through the extracellular signal-regulated kinase pathway

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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