TY - JOUR
T1 - Sperm mRNA screening by reverse transcription-recombinase polymerase amplification (RT-RPA) assay
T2 - Decision-making for differential extraction
AU - Kubo, Seiji
AU - Niimi, Hideki
AU - Kitajima, Isao
N1 - Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - The presence of sperm cells is an indicator for differential extraction on sexual assault samples. In general, sperm cells are identified by microscopic analysis; however, this conventional method takes time and effort, even for trained personnel. Here, we present a reverse transcription-recombinase polymerase amplification (RT-RPA) assay targeting sperm mRNA marker (PRM1). The RT-RPA assay requires only 40 min for PRM1 detection and demonstrates a sensitivity of 0.1 μL of semen. Our results indicate that the RT-RPA assay may be a rapid, simple, and specific strategy for screening sperm cells in sexual assault samples.
AB - The presence of sperm cells is an indicator for differential extraction on sexual assault samples. In general, sperm cells are identified by microscopic analysis; however, this conventional method takes time and effort, even for trained personnel. Here, we present a reverse transcription-recombinase polymerase amplification (RT-RPA) assay targeting sperm mRNA marker (PRM1). The RT-RPA assay requires only 40 min for PRM1 detection and demonstrates a sensitivity of 0.1 μL of semen. Our results indicate that the RT-RPA assay may be a rapid, simple, and specific strategy for screening sperm cells in sexual assault samples.
KW - Forensic science
KW - Protamine 1
KW - Recombinase polymerase amplification
KW - Sperm mRNA
UR - http://www.scopus.com/inward/record.url?scp=85151259571&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2023.115121
DO - 10.1016/j.ab.2023.115121
M3 - 学術論文
C2 - 36972745
AN - SCOPUS:85151259571
SN - 0003-2697
VL - 670
JO - Analytical Biochemistry
JF - Analytical Biochemistry
M1 - 115121
ER -