Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments

Akinori Wada; Aya Ito; Hirofumi Iitsuka; Koichi Tsuneyama; Takayoshi Miyazono; Jun Murakami; Naotoshi Shibahara; Hiroaki Sakurai; Ikuo Saiki; Takashi Nakayama; Osamu Yoshie; Keiichi Koizumi; Toshiro Sugiyama

doi:10.3892/or.2015.3941

Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments

Akinori Wada, Aya Ito, Hirofumi Iitsuka, Koichi Tsuneyama, Takayoshi Miyazono, Jun Murakami, Naotoshi Shibahara, Hiroaki Sakurai, Ikuo Saiki, Takashi Nakayama, Osamu Yoshie, Keiichi Koizumi^*, Toshiro Sugiyama

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Several chemokines/chemokine receptors such as CXCL12, CCL3, CXCR4 and CCR1 attract multiple myelomas to specific microenvironments. In the present study, we investigated whether the CX3CL1/CX3CR1 axis is involved in the interaction of the multiple myeloma cells with their microenvironment. The expression of CX3CR1 (also known as fractalkine) was detected in three of the seven human myeloma cell lines. CX3CL1-induced phosphorylation of Akt and ERK1/2 was detected in the CX3CR1-positive cell lines, but not in the CX3CR1-negative cell lines. In addition, CX3CL1-induced cell adhesion to fibronectin and vascular cell adhesion molecule-1 (VCAM-1) in the human myeloma RPMI-8226 cell line. We also investigated whether a relationship existed between myeloma cells and osteoclasts that may function via the CX3CL1/CX3CR1 axis. Conditioned medium from CX3CL1-stimulated RPMI-8226 cells drastically increased the osteoclast differentiation. Collectively, the results from the present study support the concept of the CX3CL1-mediated activation of the progression of the multiple myeloma via CX3CR1. Thus, CX3CR1 may represent a potential therapeutic target for the treatment of multiple myeloma in a bone microenvironment.

Original language	English
Pages (from-to)	2935-2939
Number of pages	5
Journal	Oncology Reports
Volume	33
Issue number	6
DOIs	https://doi.org/10.3892/or.2015.3941
State	Published - 2015/06/01

Keywords

Adhesion
Chemokine cx3cl1
Multiple myeloma
Osteoclast
Survival pathway

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments",

abstract = "Several chemokines/chemokine receptors such as CXCL12, CCL3, CXCR4 and CCR1 attract multiple myelomas to specific microenvironments. In the present study, we investigated whether the CX3CL1/CX3CR1 axis is involved in the interaction of the multiple myeloma cells with their microenvironment. The expression of CX3CR1 (also known as fractalkine) was detected in three of the seven human myeloma cell lines. CX3CL1-induced phosphorylation of Akt and ERK1/2 was detected in the CX3CR1-positive cell lines, but not in the CX3CR1-negative cell lines. In addition, CX3CL1-induced cell adhesion to fibronectin and vascular cell adhesion molecule-1 (VCAM-1) in the human myeloma RPMI-8226 cell line. We also investigated whether a relationship existed between myeloma cells and osteoclasts that may function via the CX3CL1/CX3CR1 axis. Conditioned medium from CX3CL1-stimulated RPMI-8226 cells drastically increased the osteoclast differentiation. Collectively, the results from the present study support the concept of the CX3CL1-mediated activation of the progression of the multiple myeloma via CX3CR1. Thus, CX3CR1 may represent a potential therapeutic target for the treatment of multiple myeloma in a bone microenvironment.",

keywords = "Adhesion, Chemokine cx3cl1, Multiple myeloma, Osteoclast, Survival pathway",

author = "Akinori Wada and Aya Ito and Hirofumi Iitsuka and Koichi Tsuneyama and Takayoshi Miyazono and Jun Murakami and Naotoshi Shibahara and Hiroaki Sakurai and Ikuo Saiki and Takashi Nakayama and Osamu Yoshie and Keiichi Koizumi and Toshiro Sugiyama",

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doi = "10.3892/or.2015.3941",

language = "英語",

volume = "33",

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T1 - Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments

AU - Wada, Akinori

AU - Ito, Aya

AU - Iitsuka, Hirofumi

AU - Tsuneyama, Koichi

AU - Miyazono, Takayoshi

AU - Murakami, Jun

AU - Shibahara, Naotoshi

AU - Sakurai, Hiroaki

AU - Saiki, Ikuo

AU - Nakayama, Takashi

AU - Yoshie, Osamu

AU - Koizumi, Keiichi

AU - Sugiyama, Toshiro

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Several chemokines/chemokine receptors such as CXCL12, CCL3, CXCR4 and CCR1 attract multiple myelomas to specific microenvironments. In the present study, we investigated whether the CX3CL1/CX3CR1 axis is involved in the interaction of the multiple myeloma cells with their microenvironment. The expression of CX3CR1 (also known as fractalkine) was detected in three of the seven human myeloma cell lines. CX3CL1-induced phosphorylation of Akt and ERK1/2 was detected in the CX3CR1-positive cell lines, but not in the CX3CR1-negative cell lines. In addition, CX3CL1-induced cell adhesion to fibronectin and vascular cell adhesion molecule-1 (VCAM-1) in the human myeloma RPMI-8226 cell line. We also investigated whether a relationship existed between myeloma cells and osteoclasts that may function via the CX3CL1/CX3CR1 axis. Conditioned medium from CX3CL1-stimulated RPMI-8226 cells drastically increased the osteoclast differentiation. Collectively, the results from the present study support the concept of the CX3CL1-mediated activation of the progression of the multiple myeloma via CX3CR1. Thus, CX3CR1 may represent a potential therapeutic target for the treatment of multiple myeloma in a bone microenvironment.

AB - Several chemokines/chemokine receptors such as CXCL12, CCL3, CXCR4 and CCR1 attract multiple myelomas to specific microenvironments. In the present study, we investigated whether the CX3CL1/CX3CR1 axis is involved in the interaction of the multiple myeloma cells with their microenvironment. The expression of CX3CR1 (also known as fractalkine) was detected in three of the seven human myeloma cell lines. CX3CL1-induced phosphorylation of Akt and ERK1/2 was detected in the CX3CR1-positive cell lines, but not in the CX3CR1-negative cell lines. In addition, CX3CL1-induced cell adhesion to fibronectin and vascular cell adhesion molecule-1 (VCAM-1) in the human myeloma RPMI-8226 cell line. We also investigated whether a relationship existed between myeloma cells and osteoclasts that may function via the CX3CL1/CX3CR1 axis. Conditioned medium from CX3CL1-stimulated RPMI-8226 cells drastically increased the osteoclast differentiation. Collectively, the results from the present study support the concept of the CX3CL1-mediated activation of the progression of the multiple myeloma via CX3CR1. Thus, CX3CR1 may represent a potential therapeutic target for the treatment of multiple myeloma in a bone microenvironment.

KW - Adhesion

KW - Chemokine cx3cl1

KW - Multiple myeloma

KW - Osteoclast

KW - Survival pathway

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JO - Oncology Reports

JF - Oncology Reports

IS - 6

ER -

Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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