Protective effects of platinum nanoparticles against UV-light-induced epidermal inflammation

Yoko Yoshihisa; Ayumi Honda; Qing Li Zhao; Teruhiko Makino; Riichiro Abe; Kotaro Matsui; Hiroshi Shimizu; Yusei Miyamoto; Takashi Kondo; Tadamichi Shimizu

doi:10.1111/j.1600-0625.2010.01128.x

Protective effects of platinum nanoparticles against UV-light-induced epidermal inflammation

Yoko Yoshihisa, Ayumi Honda, Qing Li Zhao, Teruhiko Makino, Riichiro Abe, Kotaro Matsui, Hiroshi Shimizu, Yusei Miyamoto, Takashi Kondo, Tadamichi Shimizu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Intracellular reactive oxygen species (ROS) and apoptosis play important roles in the ultraviolet (UV)-induced inflammatory responses in the skin. Metal nanoparticles have been developed to increase the catalytic activity of metals, which is because of the large surface area of smaller particles. Platinum nanoparticles (nano-Pt) protected by poly acrylic acid were manufactured by reduction with ethanol. A marked increase in ROS production was observed in UV-treated HaCaT keratinocytes cell lines, while a decrease in ROS production was observed in nano-Pt-treated cells. Pretreatment of the cells with nano-Pt also caused a significant inhibition of UVB- and UVC-induced apoptosis. Furthermore, we found that mice treated with nano-Pt gel prior to UV irradiation showed significant inhibition of UVB-induced inflammation and UVA-induced photoallergy compared to UV-irradiated control mice. These results suggest that nano-Pt effectively protects against UV-induced inflammation by decreasing ROS production and inhibiting apoptosis in keratinocytes.

Original language	English
Pages (from-to)	1000-1006
Number of pages	7
Journal	Experimental Dermatology
Volume	19
Issue number	11
DOIs	https://doi.org/10.1111/j.1600-0625.2010.01128.x
State	Published - 2010/11

Keywords

Apoptosis
Platinum nanoparticles
Reactive oxygen species
Skin
Ultraviolet

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Dermatology

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10.1111/j.1600-0625.2010.01128.x

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@article{451da67d3940434db3a063aba161f167,

title = "Protective effects of platinum nanoparticles against UV-light-induced epidermal inflammation",

abstract = "Intracellular reactive oxygen species (ROS) and apoptosis play important roles in the ultraviolet (UV)-induced inflammatory responses in the skin. Metal nanoparticles have been developed to increase the catalytic activity of metals, which is because of the large surface area of smaller particles. Platinum nanoparticles (nano-Pt) protected by poly acrylic acid were manufactured by reduction with ethanol. A marked increase in ROS production was observed in UV-treated HaCaT keratinocytes cell lines, while a decrease in ROS production was observed in nano-Pt-treated cells. Pretreatment of the cells with nano-Pt also caused a significant inhibition of UVB- and UVC-induced apoptosis. Furthermore, we found that mice treated with nano-Pt gel prior to UV irradiation showed significant inhibition of UVB-induced inflammation and UVA-induced photoallergy compared to UV-irradiated control mice. These results suggest that nano-Pt effectively protects against UV-induced inflammation by decreasing ROS production and inhibiting apoptosis in keratinocytes.",

keywords = "Apoptosis, Platinum nanoparticles, Reactive oxygen species, Skin, Ultraviolet",

author = "Yoko Yoshihisa and Ayumi Honda and Zhao, {Qing Li} and Teruhiko Makino and Riichiro Abe and Kotaro Matsui and Hiroshi Shimizu and Yusei Miyamoto and Takashi Kondo and Tadamichi Shimizu",

year = "2010",

month = nov,

doi = "10.1111/j.1600-0625.2010.01128.x",

language = "英語",

volume = "19",

pages = "1000--1006",

journal = "Experimental Dermatology",

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publisher = "John Wiley and Sons Inc.",

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AU - Yoshihisa, Yoko

AU - Honda, Ayumi

AU - Zhao, Qing Li

AU - Makino, Teruhiko

AU - Abe, Riichiro

AU - Matsui, Kotaro

AU - Shimizu, Hiroshi

AU - Miyamoto, Yusei

AU - Kondo, Takashi

AU - Shimizu, Tadamichi

PY - 2010/11

Y1 - 2010/11

N2 - Intracellular reactive oxygen species (ROS) and apoptosis play important roles in the ultraviolet (UV)-induced inflammatory responses in the skin. Metal nanoparticles have been developed to increase the catalytic activity of metals, which is because of the large surface area of smaller particles. Platinum nanoparticles (nano-Pt) protected by poly acrylic acid were manufactured by reduction with ethanol. A marked increase in ROS production was observed in UV-treated HaCaT keratinocytes cell lines, while a decrease in ROS production was observed in nano-Pt-treated cells. Pretreatment of the cells with nano-Pt also caused a significant inhibition of UVB- and UVC-induced apoptosis. Furthermore, we found that mice treated with nano-Pt gel prior to UV irradiation showed significant inhibition of UVB-induced inflammation and UVA-induced photoallergy compared to UV-irradiated control mice. These results suggest that nano-Pt effectively protects against UV-induced inflammation by decreasing ROS production and inhibiting apoptosis in keratinocytes.

AB - Intracellular reactive oxygen species (ROS) and apoptosis play important roles in the ultraviolet (UV)-induced inflammatory responses in the skin. Metal nanoparticles have been developed to increase the catalytic activity of metals, which is because of the large surface area of smaller particles. Platinum nanoparticles (nano-Pt) protected by poly acrylic acid were manufactured by reduction with ethanol. A marked increase in ROS production was observed in UV-treated HaCaT keratinocytes cell lines, while a decrease in ROS production was observed in nano-Pt-treated cells. Pretreatment of the cells with nano-Pt also caused a significant inhibition of UVB- and UVC-induced apoptosis. Furthermore, we found that mice treated with nano-Pt gel prior to UV irradiation showed significant inhibition of UVB-induced inflammation and UVA-induced photoallergy compared to UV-irradiated control mice. These results suggest that nano-Pt effectively protects against UV-induced inflammation by decreasing ROS production and inhibiting apoptosis in keratinocytes.

KW - Apoptosis

KW - Platinum nanoparticles

KW - Reactive oxygen species

KW - Skin

KW - Ultraviolet

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JO - Experimental Dermatology

JF - Experimental Dermatology

IS - 11

ER -

Protective effects of platinum nanoparticles against UV-light-induced epidermal inflammation

Abstract

Keywords

ASJC Scopus subject areas

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