Abstract
Paternal antigen-specific regulatory T (PA-specific Treg) cells play an important role in feto-maternal tolerance. To detect the PA-specific Tregs, female BALB/c mice were mated with male DBA/2 mice. Mls Ia antigen on DBA/2 mice is recognized by the T-cell receptor Vβ6; thus, CD4+Foxp3+Vβ6+ cells are recognized as PA-specific Treg cells. CD4+CD25+Vβ6+ cells effectively suppressed the allo-reactive proliferation of lymphocytes compared with that of CD4+CD25+Vβ6- cells. Vβ6+ PA-specific Treg cells expressed CCR4 and CCR5 on their surface. The frequency of Ki67+ PA-specific Treg cells among Treg cells was significantly increased in draining lymph nodes on day 3.5 post-coitus (pc; 6.8 ± 1.1%, p<0.05) and day 5.5 pc (7.2 ± 1.1%, p<0.05) in allogeneic pregnant mice compared with that in nonpregnant mice (2.7 ± 0.2%). The frequency of Ki67+ PA-specific Treg cells in the uterus increased significantly after day 5.5 pc in allogeneic pregnant mice compared with that in nonpregnant mice (8.8 ± 2.8% vs. 1.2 ± 1.3%, p<0.05). However, Ki67-PA-specific Tregs did not change during pregnancy. To analyze the role of seminal fluid or sperm in Treg expansion, female BALB/c mice were mated with vasectomized DBA/2 male mice (VAS) or seminal vesicle-excised DBA/2 male mice (SVX). The frequency of Ki67+ PA-specific Treg cells did not increase in draining lymph nodes or uterus in BALB/c×DBA/2 (SVX) allogeneic mating mice. These findings suggest that the priming by seminal fluid is important for the induction of proliferating PA-specific Tregs in uterine-draining lymph nodes just before implantation and pregnant uterus after implantation, resulting in successful implantation and the maintenance of allogeneic pregnancy.
Original language | English |
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Pages (from-to) | 72-82 |
Number of pages | 11 |
Journal | Journal of Reproductive Immunology |
Volume | 108 |
DOIs | |
State | Published - 2015/04/01 |
Keywords
- Chemokine receptor
- Feto-maternal tolerance
- Paternal antigen
- Regulatory T cells
- Seminal fluid
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Reproductive Medicine
- Obstetrics and Gynecology