TY - JOUR
T1 - mRNA differential display reveals Krox-20 as a neural plasticity-regulated gene in the rat hippocampus
AU - Inokuchi, Kaoru
AU - Murayama, Akiko
AU - Ozawa, Fumiko
N1 - Funding Information:
We thank K. Hirai for technical assistance with construction of cDNA library, M. Inoue for technical suggestions and A. Kato for helpful discussions. This work was supported in part by Grant in Aid for Scientific Research on Priority Areas on “Functional Development of Neural Circuits”, the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1996/4/16
Y1 - 1996/4/16
N2 - The prolonged maintenance of hippocampal long-term potentiation depends on de novo protein and RNA synthesis, indicating an involvement of altered gene expression in long-lasting plastic changes in synaptic efficacy. We have used an mRNA differential display technique to identify a set of genes that are induced by neural activity in the rat hippocampus. Sixteen independent cDNAs were isolated whose mRNA level was markedly modulated by convulsive seizure. One of these encodes Krox-20, a zinc finger DNA binding protein. High frequency tetanic stimulation of perforant pathway, which elicited a persistent long-term potentiation (> 10 h), rapidly induced expression of krox-20 mRNA in the hippocampus of urethane-anesthetized rat. The increase in krox-20 mRNA was transient and NMDA receptor-dependent. These results suggest a role for krox-20 in the maintenance of long-term potentiation.
AB - The prolonged maintenance of hippocampal long-term potentiation depends on de novo protein and RNA synthesis, indicating an involvement of altered gene expression in long-lasting plastic changes in synaptic efficacy. We have used an mRNA differential display technique to identify a set of genes that are induced by neural activity in the rat hippocampus. Sixteen independent cDNAs were isolated whose mRNA level was markedly modulated by convulsive seizure. One of these encodes Krox-20, a zinc finger DNA binding protein. High frequency tetanic stimulation of perforant pathway, which elicited a persistent long-term potentiation (> 10 h), rapidly induced expression of krox-20 mRNA in the hippocampus of urethane-anesthetized rat. The increase in krox-20 mRNA was transient and NMDA receptor-dependent. These results suggest a role for krox-20 in the maintenance of long-term potentiation.
UR - http://www.scopus.com/inward/record.url?scp=0030003799&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0612
DO - 10.1006/bbrc.1996.0612
M3 - 学術論文
C2 - 8619872
AN - SCOPUS:0030003799
SN - 0006-291X
VL - 221
SP - 430
EP - 436
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -