Abstract
Mycolic acid-containing bacteria (MACB) are known to activate cryptic natural product biosynthesis in co-cultures with actinobacteria. We cultured Actinosynnema mirum NBRC 14064, a producer of the mono-cyclic polyene macrolactam mirilactam A (6), with the MACB Tsukamurella pulmonis TP-B0596. As a result, three novel compounds (mirilactams C–E, 1–3) were produced in the co-culture conditions. Compounds 1–3 were likely derived from 6 by epoxidation and subsequent spontaneous cyclization. The chemical structures and stereochemistries of 1–3 were determined by spectroscopic analyses (NMR and MS), conformational searches in the optimized potentials for liquid simulations-3 (OPLS3) force field, and calculations of electronic circular dichroism (ECD).
Original language | English |
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Pages (from-to) | 660-667 |
Number of pages | 8 |
Journal | Chemical and Pharmaceutical Bulletin |
Volume | 66 |
Issue number | 6 |
DOIs | |
State | Published - 2018 |
Keywords
- Combined-culture
- Mycolic acid-containing bacterium
- Polyene macrolactam
ASJC Scopus subject areas
- General Chemistry
- Drug Discovery