TY - JOUR
T1 - Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
AU - Kubo, Yoshiyuki
AU - Ishizuka, Sakiko
AU - Ito, Takeru
AU - Yoneyama, Daisuke
AU - Akanuma, Shin Ichi
AU - Hosoya, Ken Ichi
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/1
Y1 - 2022/1
N2 - Taurine transport was investigated at the blood–testis barrier (BTB) formed by Sertoli cells. An integration plot analysis of mice showed the apparent influx permeability clearance of [3 H]taurine (27.7 µL/(min·g testis)), which was much higher than that of a non-permeable paracellular marker, suggesting blood-to-testis transport of taurine, which may involve a facilitative taurine transport system at the BTB. A mouse Sertoli cell line, TM4 cells, showed temperature-and concentrationdependent [3 H]taurine uptake with a Km of 13.5 µM, suggesting that the influx transport of taurine at the BTB involves a carrier-mediated process. [3 H]Taurine uptake by TM4 cells was significantly reduced by the substrates of taurine transporter (TauT/SLC6A6), such as β-alanine, hypotaurine, γ-aminobutyric acid (GABA), and guanidinoacetic acid (GAA), with no significant effect shown by L-alanine, probenecid, and L-leucine. In addition, the concentration-dependent inhibition of [3 H]taurine uptake revealed an IC50 of 378 µM for GABA. Protein expression of TauT in the testis, seminiferous tubules, and TM4 cells was confirmed by Western blot analysis and immunohistochemistry by means of anti-TauT antibodies, and knockdown of TauT showed significantly decreased [3 H]taurine uptake by TM4 cells. These results suggest the involvement of TauT in the transport of taurine at the BTB.
AB - Taurine transport was investigated at the blood–testis barrier (BTB) formed by Sertoli cells. An integration plot analysis of mice showed the apparent influx permeability clearance of [3 H]taurine (27.7 µL/(min·g testis)), which was much higher than that of a non-permeable paracellular marker, suggesting blood-to-testis transport of taurine, which may involve a facilitative taurine transport system at the BTB. A mouse Sertoli cell line, TM4 cells, showed temperature-and concentrationdependent [3 H]taurine uptake with a Km of 13.5 µM, suggesting that the influx transport of taurine at the BTB involves a carrier-mediated process. [3 H]Taurine uptake by TM4 cells was significantly reduced by the substrates of taurine transporter (TauT/SLC6A6), such as β-alanine, hypotaurine, γ-aminobutyric acid (GABA), and guanidinoacetic acid (GAA), with no significant effect shown by L-alanine, probenecid, and L-leucine. In addition, the concentration-dependent inhibition of [3 H]taurine uptake revealed an IC50 of 378 µM for GABA. Protein expression of TauT in the testis, seminiferous tubules, and TM4 cells was confirmed by Western blot analysis and immunohistochemistry by means of anti-TauT antibodies, and knockdown of TauT showed significantly decreased [3 H]taurine uptake by TM4 cells. These results suggest the involvement of TauT in the transport of taurine at the BTB.
KW - Antioxidant
KW - Blood-testis barrier
KW - Infertility
KW - Seminiferous tubules
KW - Taurine
KW - Transport
UR - http://www.scopus.com/inward/record.url?scp=85123168206&partnerID=8YFLogxK
U2 - 10.3390/metabo12010066
DO - 10.3390/metabo12010066
M3 - 学術論文
C2 - 35050188
AN - SCOPUS:85123168206
SN - 2218-1989
VL - 12
JO - Metabolites
JF - Metabolites
IS - 1
M1 - 66
ER -