IL-10 production in mouse hepatocytes augmented by TGF-β

Shigeaki Ishizaka; Shigeru Saito; Masahide Yoshikawa; Makoto Kimoto; Toshimasa Nishiyama

doi:10.1006/cyto.1996.0112

IL-10 production in mouse hepatocytes augmented by TGF-β

Shigeaki Ishizaka^*, Shigeru Saito, Masahide Yoshikawa, Makoto Kimoto, Toshimasa Nishiyama

^*Corresponding author for this work

Member of the Board

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-β)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared with the untreated supernatants. IL-10 production and expression of mRNA for IL-10 in Clone 1469 cells was detected by using enzyme-linked immunosorbent assay, ELISPOT assay and PCR amplification. TGF-β led to augmentation of IL-10 secretion from Clone 1469 cells.

Original language	English
Pages (from-to)	837-843
Number of pages	7
Journal	Cytokine
Volume	8
Issue number	11
DOIs	https://doi.org/10.1006/cyto.1996.0112
State	Published - 1996/11

Keywords

Antibody production
Hepatocyte
IL-10
TGF-β

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Biochemistry
Molecular Biology
Hematology

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10.1006/cyto.1996.0112

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title = "IL-10 production in mouse hepatocytes augmented by TGF-β",

abstract = "The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-β)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared with the untreated supernatants. IL-10 production and expression of mRNA for IL-10 in Clone 1469 cells was detected by using enzyme-linked immunosorbent assay, ELISPOT assay and PCR amplification. TGF-β led to augmentation of IL-10 secretion from Clone 1469 cells.",

keywords = "Antibody production, Hepatocyte, IL-10, TGF-β",

author = "Shigeaki Ishizaka and Shigeru Saito and Masahide Yoshikawa and Makoto Kimoto and Toshimasa Nishiyama",

note = "Funding Information: This study was supported in part by Grant!in Aids for Scienti_c Research from the Japanese Ministry of Education\ Science and Culture {"}95560564# and by the Health Science Foundation[",

year = "1996",

month = nov,

doi = "10.1006/cyto.1996.0112",

language = "英語",

volume = "8",

pages = "837--843",

journal = "Cytokine",

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TY - JOUR

T1 - IL-10 production in mouse hepatocytes augmented by TGF-β

AU - Ishizaka, Shigeaki

AU - Saito, Shigeru

AU - Yoshikawa, Masahide

AU - Kimoto, Makoto

AU - Nishiyama, Toshimasa

N1 - Funding Information: This study was supported in part by Grant!in Aids for Scienti_c Research from the Japanese Ministry of Education\ Science and Culture "95560564# and by the Health Science Foundation[

PY - 1996/11

Y1 - 1996/11

N2 - The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-β)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared with the untreated supernatants. IL-10 production and expression of mRNA for IL-10 in Clone 1469 cells was detected by using enzyme-linked immunosorbent assay, ELISPOT assay and PCR amplification. TGF-β led to augmentation of IL-10 secretion from Clone 1469 cells.

AB - The supernatant from normal liver cell (Clone 1469) culture enhanced polyclonal antibody production in mouse spleen cells but not in human peripheral blood lymphocytes. The supernatants from transforming growth factor beta (TGF-β)-treated C3H/HeN spleen cells augmented greatly polyclonal antibody responses compared with the untreated supernatants. IL-10 production and expression of mRNA for IL-10 in Clone 1469 cells was detected by using enzyme-linked immunosorbent assay, ELISPOT assay and PCR amplification. TGF-β led to augmentation of IL-10 secretion from Clone 1469 cells.

KW - Antibody production

KW - Hepatocyte

KW - IL-10

KW - TGF-β

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U2 - 10.1006/cyto.1996.0112

DO - 10.1006/cyto.1996.0112

M3 - 学術論文

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AN - SCOPUS:0030295035

SN - 1043-4666

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SP - 837

EP - 843

JO - Cytokine

JF - Cytokine

IS - 11

ER -

IL-10 production in mouse hepatocytes augmented by TGF-β

Abstract

Keywords

ASJC Scopus subject areas

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