Helodermin has a VIP-like effect upon canine blood flow

S. Naruse; A. Yasui; S. Kishida; M. Kadowaki; M. Hoshino; T. Ozaki; P. Robberecht; J. Christophe; C. Yanaihara; N. Yanaihara

doi:10.1016/0196-9781(86)90192-0

Helodermin has a VIP-like effect upon canine blood flow

S. Naruse, A. Yasui, S. Kishida, M. Kadowaki, M. Hoshino, T. Ozaki, P. Robberecht, J. Christophe, C. Yanaihara, N. Yanaihara^*

^*Corresponding author for this work

Research Center for Pre-Disease Science

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The effect of helodermin on vascular physiology was studied in anesthetized dogs using a synthetic replicate of helodermin and helodermin related peptides. Intraarterial infusion of helodermin caused a dosedependent increase in femoral blood flow. Helodermin was 16 times less potent than VIP and 5 times more potent than PHM (human PHI). The helodermin effect lasted significantly longer; the half-life of the helodermin effect was 6.5 times longer than VIP. Synthetic helodermin (Hd) N-terminal fragment Hd(1-27)NH₂ retained substantial activity similar to the full helodermin molecule but the prolonged effect was lost. Hd(7-35) and Hd(22-35) were inactive in this system. Intravenous injection of synthetic helodermin produced prolonged systemic hypotension and tachycardia; and, similar to VIP, it increased the common carotid arterial blood flow while those of the superior mesenteric and femoral arteries were decreased. The results demonstrate the VIP-like vasodilating activity and cardiovascular effects of helodermin in anesthetized dogs.

Original language	English
Pages (from-to)	237-240
Number of pages	4
Journal	Peptides
Volume	7
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/0196-9781(86)90192-0
State	Published - 1986

Keywords

Femoral blood flow
Helodermin
Peptide histidine isoleucine/methionine (PHI/PHM)
Structure-function relationship
Vasoactive intestinal polypeptide (VIP)

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(86)90192-0

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title = "Helodermin has a VIP-like effect upon canine blood flow",

abstract = "The effect of helodermin on vascular physiology was studied in anesthetized dogs using a synthetic replicate of helodermin and helodermin related peptides. Intraarterial infusion of helodermin caused a dosedependent increase in femoral blood flow. Helodermin was 16 times less potent than VIP and 5 times more potent than PHM (human PHI). The helodermin effect lasted significantly longer; the half-life of the helodermin effect was 6.5 times longer than VIP. Synthetic helodermin (Hd) N-terminal fragment Hd(1-27)NH2 retained substantial activity similar to the full helodermin molecule but the prolonged effect was lost. Hd(7-35) and Hd(22-35) were inactive in this system. Intravenous injection of synthetic helodermin produced prolonged systemic hypotension and tachycardia; and, similar to VIP, it increased the common carotid arterial blood flow while those of the superior mesenteric and femoral arteries were decreased. The results demonstrate the VIP-like vasodilating activity and cardiovascular effects of helodermin in anesthetized dogs.",

keywords = "Femoral blood flow, Helodermin, Peptide histidine isoleucine/methionine (PHI/PHM), Structure-function relationship, Vasoactive intestinal polypeptide (VIP)",

author = "S. Naruse and A. Yasui and S. Kishida and M. Kadowaki and M. Hoshino and T. Ozaki and P. Robberecht and J. Christophe and C. Yanaihara and N. Yanaihara",

note = "Funding Information: This work was supportedin part by Grants-in-Aidfr om the Ministryo f EducationS, ciencea ndC ulturef or SpeciaPl rojectR e-searchN o. 5922700a5n d6 022900a5n df or CooperativeR esearch No. 5937000a5n d Grants-in-Aidfr omthe Ministryo f Healtha nd Welfaref or CancerR esearchN o. 56-6.",

year = "1986",

doi = "10.1016/0196-9781(86)90192-0",

language = "英語",

volume = "7",

pages = "237--240",

journal = "Peptides",

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publisher = "Elsevier Inc.",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Helodermin has a VIP-like effect upon canine blood flow

AU - Naruse, S.

AU - Yasui, A.

AU - Kishida, S.

AU - Kadowaki, M.

AU - Hoshino, M.

AU - Ozaki, T.

AU - Robberecht, P.

AU - Christophe, J.

AU - Yanaihara, C.

AU - Yanaihara, N.

N1 - Funding Information: This work was supportedin part by Grants-in-Aidfr om the Ministryo f EducationS, ciencea ndC ulturef or SpeciaPl rojectR e-searchN o. 5922700a5n d6 022900a5n df or CooperativeR esearch No. 5937000a5n d Grants-in-Aidfr omthe Ministryo f Healtha nd Welfaref or CancerR esearchN o. 56-6.

PY - 1986

Y1 - 1986

N2 - The effect of helodermin on vascular physiology was studied in anesthetized dogs using a synthetic replicate of helodermin and helodermin related peptides. Intraarterial infusion of helodermin caused a dosedependent increase in femoral blood flow. Helodermin was 16 times less potent than VIP and 5 times more potent than PHM (human PHI). The helodermin effect lasted significantly longer; the half-life of the helodermin effect was 6.5 times longer than VIP. Synthetic helodermin (Hd) N-terminal fragment Hd(1-27)NH2 retained substantial activity similar to the full helodermin molecule but the prolonged effect was lost. Hd(7-35) and Hd(22-35) were inactive in this system. Intravenous injection of synthetic helodermin produced prolonged systemic hypotension and tachycardia; and, similar to VIP, it increased the common carotid arterial blood flow while those of the superior mesenteric and femoral arteries were decreased. The results demonstrate the VIP-like vasodilating activity and cardiovascular effects of helodermin in anesthetized dogs.

AB - The effect of helodermin on vascular physiology was studied in anesthetized dogs using a synthetic replicate of helodermin and helodermin related peptides. Intraarterial infusion of helodermin caused a dosedependent increase in femoral blood flow. Helodermin was 16 times less potent than VIP and 5 times more potent than PHM (human PHI). The helodermin effect lasted significantly longer; the half-life of the helodermin effect was 6.5 times longer than VIP. Synthetic helodermin (Hd) N-terminal fragment Hd(1-27)NH2 retained substantial activity similar to the full helodermin molecule but the prolonged effect was lost. Hd(7-35) and Hd(22-35) were inactive in this system. Intravenous injection of synthetic helodermin produced prolonged systemic hypotension and tachycardia; and, similar to VIP, it increased the common carotid arterial blood flow while those of the superior mesenteric and femoral arteries were decreased. The results demonstrate the VIP-like vasodilating activity and cardiovascular effects of helodermin in anesthetized dogs.

KW - Femoral blood flow

KW - Helodermin

KW - Peptide histidine isoleucine/methionine (PHI/PHM)

KW - Structure-function relationship

KW - Vasoactive intestinal polypeptide (VIP)

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U2 - 10.1016/0196-9781(86)90192-0

DO - 10.1016/0196-9781(86)90192-0

M3 - 学術論文

C2 - 3748849

AN - SCOPUS:0022511984

SN - 0196-9781

VL - 7

SP - 237

EP - 240

JO - Peptides

JF - Peptides

IS - SUPPL. 1

ER -

Helodermin has a VIP-like effect upon canine blood flow

Abstract

Keywords

ASJC Scopus subject areas

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