Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells

Hiraku Motomura; Hideki Niimi; Kazuhito Sugimori; Toshihisa Ohtsuka; Tomoatsu Kimura; Isao Kitajima

doi:10.1016/j.bbrc.2007.04.035

Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells

Hiraku Motomura, Hideki Niimi, Kazuhito Sugimori, Toshihisa Ohtsuka, Tomoatsu Kimura, Isao Kitajima^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The mesenchymal cell line C3H10T1/2 can be preferentially induced toward chondrogenesis by culturing as a micromass in the presence of bone morphogenetic protein 2. To screen new regulator genes for chondrogenic differentiation, we performed differential display polymerase chain reaction and identified growth arrest-specific 6 (Gas6) as a gene that was clearly downregulated by this induction of chondrogenic differentiation. Blockage of Gas6 mRNA expression by siRNA remarkably enhanced the chondrogenic differentiation, while stimulation with recombinant Gas6 inhibited the mRNA expressions of type II collagen (Col2a1) and aggrecan. Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway.

Original language	English
Pages (from-to)	997-1003
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2007.04.035
State	Published - 2007/06/15

Keywords

C3H10T1/2 cell
Chondrogenesis
Differentiation
Gas6
MAPK

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.04.035

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title = "Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells",

abstract = "The mesenchymal cell line C3H10T1/2 can be preferentially induced toward chondrogenesis by culturing as a micromass in the presence of bone morphogenetic protein 2. To screen new regulator genes for chondrogenic differentiation, we performed differential display polymerase chain reaction and identified growth arrest-specific 6 (Gas6) as a gene that was clearly downregulated by this induction of chondrogenic differentiation. Blockage of Gas6 mRNA expression by siRNA remarkably enhanced the chondrogenic differentiation, while stimulation with recombinant Gas6 inhibited the mRNA expressions of type II collagen (Col2a1) and aggrecan. Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway.",

keywords = "C3H10T1/2 cell, Chondrogenesis, Differentiation, Gas6, MAPK",

author = "Hiraku Motomura and Hideki Niimi and Kazuhito Sugimori and Toshihisa Ohtsuka and Tomoatsu Kimura and Isao Kitajima",

note = "Funding Information: We thank Astellas Pharma Inc. for the recombinant human BMP-2 and Shionogi Research Laboratory, Shionogi & Co., Ltd., for the recombinant rat Gas6. This work was supported in part by a Grant-in-Aid for the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan. ",

year = "2007",

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doi = "10.1016/j.bbrc.2007.04.035",

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T1 - Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells

AU - Motomura, Hiraku

AU - Niimi, Hideki

AU - Sugimori, Kazuhito

AU - Ohtsuka, Toshihisa

AU - Kimura, Tomoatsu

AU - Kitajima, Isao

N1 - Funding Information: We thank Astellas Pharma Inc. for the recombinant human BMP-2 and Shionogi Research Laboratory, Shionogi & Co., Ltd., for the recombinant rat Gas6. This work was supported in part by a Grant-in-Aid for the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2007/6/15

Y1 - 2007/6/15

N2 - The mesenchymal cell line C3H10T1/2 can be preferentially induced toward chondrogenesis by culturing as a micromass in the presence of bone morphogenetic protein 2. To screen new regulator genes for chondrogenic differentiation, we performed differential display polymerase chain reaction and identified growth arrest-specific 6 (Gas6) as a gene that was clearly downregulated by this induction of chondrogenic differentiation. Blockage of Gas6 mRNA expression by siRNA remarkably enhanced the chondrogenic differentiation, while stimulation with recombinant Gas6 inhibited the mRNA expressions of type II collagen (Col2a1) and aggrecan. Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway.

AB - The mesenchymal cell line C3H10T1/2 can be preferentially induced toward chondrogenesis by culturing as a micromass in the presence of bone morphogenetic protein 2. To screen new regulator genes for chondrogenic differentiation, we performed differential display polymerase chain reaction and identified growth arrest-specific 6 (Gas6) as a gene that was clearly downregulated by this induction of chondrogenic differentiation. Blockage of Gas6 mRNA expression by siRNA remarkably enhanced the chondrogenic differentiation, while stimulation with recombinant Gas6 inhibited the mRNA expressions of type II collagen (Col2a1) and aggrecan. Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway.

KW - C3H10T1/2 cell

KW - Chondrogenesis

KW - Differentiation

KW - Gas6

KW - MAPK

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AN - SCOPUS:34248233253

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells

Abstract

Keywords

ASJC Scopus subject areas

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