Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

Hiroyuki Morita; Kiyofumi Wanibuchi; Ryohei Kato; Shigetoshi Sugio; Ikuro Abe

doi:10.1107/S1744309110023572

Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

Hiroyuki Morita, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio^*, Ikuro Abe

^*Corresponding author for this work

Section of Natural Products & Drug Discovery, Division of Medicinal Resources, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxy- curcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 72.7, b = 97.2, c = 126.2 Å, = = 90.0, Β = 103.7°. A diffraction data set was collected in-house to 2.5 Å resolution.

Original language	English
Pages (from-to)	948-950
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	8
DOIs	https://doi.org/10.1107/S1744309110023572
State	Published - 2010/08/01

Keywords

chalcone synthases
curcumin synthases
curcuminoid synthases
diketide-CoA synthases
polyketide synthases

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309110023572

Cite this

@article{189e7cae61684876b0aee17ddc26b262,

title = "Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids",

abstract = "Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxy- curcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 72.7, b = 97.2, c = 126.2 {\AA}, = = 90.0, Β = 103.7°. A diffraction data set was collected in-house to 2.5 {\AA} resolution.",

keywords = "chalcone synthases, curcumin synthases, curcuminoid synthases, diketide-CoA synthases, polyketide synthases",

author = "Hiroyuki Morita and Kiyofumi Wanibuchi and Ryohei Kato and Shigetoshi Sugio and Ikuro Abe",

year = "2010",

month = aug,

day = "1",

doi = "10.1107/S1744309110023572",

language = "英語",

volume = "66",

pages = "948--950",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "8",

}

Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids. / Morita, Hiroyuki; Wanibuchi, Kiyofumi; Kato, Ryohei et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 8, 01.08.2010, p. 948-950.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

AU - Morita, Hiroyuki

AU - Wanibuchi, Kiyofumi

AU - Kato, Ryohei

AU - Sugio, Shigetoshi

AU - Abe, Ikuro

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxy- curcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 72.7, b = 97.2, c = 126.2 Å, = = 90.0, Β = 103.7°. A diffraction data set was collected in-house to 2.5 Å resolution.

AB - Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxy- curcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 72.7, b = 97.2, c = 126.2 Å, = = 90.0, Β = 103.7°. A diffraction data set was collected in-house to 2.5 Å resolution.

KW - chalcone synthases

KW - curcumin synthases

KW - curcuminoid synthases

KW - diketide-CoA synthases

KW - polyketide synthases

UR - http://www.scopus.com/inward/record.url?scp=77955450656&partnerID=8YFLogxK

U2 - 10.1107/S1744309110023572

DO - 10.1107/S1744309110023572

M3 - 学術論文

C2 - 20693677

AN - SCOPUS:77955450656

SN - 1744-3091

VL - 66

SP - 948

EP - 950

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 8

ER -

Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

Abstract

Keywords

ASJC Scopus subject areas

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