TY - JOUR
T1 - Expression of zebrafish glutamate receptor δ2 in neurons with cerebellum-like wiring
AU - Mikami, Yoshinori
AU - Yoshida, Tomoyuki
AU - Matsuda, Naoto
AU - Mishina, Masayoshi
N1 - Funding Information:
We thank Dr. Masahiko Watanabe for anti-GluRδ2 antibody. We are grateful to Dr. Ryo Kurita, Dr. Shinya Sato, and Dr. Sumiko Watanabe for helpful advice on in situ hybridization analysis and immunohistochemistry of zebrafish. This work was supported in part by research grants from the Japan Science and Technology Agency and the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2004/9/10
Y1 - 2004/9/10
N2 - Mammalian glutamate receptor (GluR) δ2 is selectively expressed in cerebellar Purkinje cells and plays key roles in cerebellar plasticity, motor learning, and neural wiring. Here, we isolated cDNA encoding the zebrafish ortholog of mammalian GluRδ2. We found that in adult zebrafish brain, glurδ2 mRNA was expressed not only in cerebellar Purkinje cells, but also in the crest cells of the medial octavolateral nucleus (MON) and the type I neurons of the optic tectum. Immunohistochemical analysis revealed that zebrafish GluRδ2 proteins were selectively localized in the apical dendrites of these neurons. Interestingly, the crest cells of the MON and the type I neurons of the optic tectum receive large numbers of parallel fiber inputs at the apical dendrites and sensory inputs at the proximal or basal dendrites. These results suggest that the expression of zebrafish GluRδ2 is selective for cerebellum-like neural wiring with large numbers of parallel fiber inputs.
AB - Mammalian glutamate receptor (GluR) δ2 is selectively expressed in cerebellar Purkinje cells and plays key roles in cerebellar plasticity, motor learning, and neural wiring. Here, we isolated cDNA encoding the zebrafish ortholog of mammalian GluRδ2. We found that in adult zebrafish brain, glurδ2 mRNA was expressed not only in cerebellar Purkinje cells, but also in the crest cells of the medial octavolateral nucleus (MON) and the type I neurons of the optic tectum. Immunohistochemical analysis revealed that zebrafish GluRδ2 proteins were selectively localized in the apical dendrites of these neurons. Interestingly, the crest cells of the MON and the type I neurons of the optic tectum receive large numbers of parallel fiber inputs at the apical dendrites and sensory inputs at the proximal or basal dendrites. These results suggest that the expression of zebrafish GluRδ2 is selective for cerebellum-like neural wiring with large numbers of parallel fiber inputs.
KW - Apical dendrite
KW - Cerebellar Purkinje cell
KW - Crest cell of the medial octavolateral nucleus
KW - Glutamate receptor δ2
KW - Neural wiring
KW - Parallel fiber
KW - Type I neuron of the optic tectum
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=4143102461&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.07.095
DO - 10.1016/j.bbrc.2004.07.095
M3 - 学術論文
C2 - 15313188
AN - SCOPUS:4143102461
SN - 0006-291X
VL - 322
SP - 168
EP - 176
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -