Engineered group i ribozymes as RNA-based modular tools to control gene expression

Yoshiya Ikawa; Shigeyoshi Matsumura

doi:10.1007/978-981-10-8372-3_13

Engineered group i ribozymes as RNA-based modular tools to control gene expression

Yoshiya Ikawa^*, Shigeyoshi Matsumura

^*Corresponding author for this work

Department of Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Group I ribozymes (RNA enzymes) constitute a class of structural RNAs that promote cleavage and ligation of phosphodiester bonds to conduct RNA splicing. As naturally occurring group I ribozymes are constitutively active in most cases, modular engineering is required to apply them to control gene expression. We first introduce exceptional examples of modified splicing reactions of group I ribozymes. We then summarize strategies to engineer group I ribozymes to develop genetic modular tools and also their application to gene expression control.

Original language	English
Title of host publication	Applied RNA Bioscience
Publisher	Springer Singapore
Pages	203-220
Number of pages	18
ISBN (Electronic)	9789811083723
ISBN (Print)	9789811083716
DOIs	https://doi.org/10.1007/978-981-10-8372-3_13
State	Published - 2018/04/10

Keywords

Alternative splicing
Aptamer
Group I intron
RNA structure
Ribozyme
Self-splicing
Trans-splicing

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Medicine

Access to Document

10.1007/978-981-10-8372-3_13

Cite this

@inbook{a0a8b2b452a44957b66389061880725e,

title = "Engineered group i ribozymes as RNA-based modular tools to control gene expression",

abstract = "Group I ribozymes (RNA enzymes) constitute a class of structural RNAs that promote cleavage and ligation of phosphodiester bonds to conduct RNA splicing. As naturally occurring group I ribozymes are constitutively active in most cases, modular engineering is required to apply them to control gene expression. We first introduce exceptional examples of modified splicing reactions of group I ribozymes. We then summarize strategies to engineer group I ribozymes to develop genetic modular tools and also their application to gene expression control.",

keywords = "Alternative splicing, Aptamer, Group I intron, RNA structure, Ribozyme, Self-splicing, Trans-splicing",

author = "Yoshiya Ikawa and Shigeyoshi Matsumura",

note = "Publisher Copyright: {\textcopyright} Springer Nature Singapore Pte Ltd. 2018.",

year = "2018",

month = apr,

day = "10",

doi = "10.1007/978-981-10-8372-3_13",

language = "英語",

isbn = "9789811083716",

pages = "203--220",

booktitle = "Applied RNA Bioscience",

publisher = "Springer Singapore",

}

TY - CHAP

T1 - Engineered group i ribozymes as RNA-based modular tools to control gene expression

AU - Ikawa, Yoshiya

AU - Matsumura, Shigeyoshi

N1 - Publisher Copyright: © Springer Nature Singapore Pte Ltd. 2018.

PY - 2018/4/10

Y1 - 2018/4/10

N2 - Group I ribozymes (RNA enzymes) constitute a class of structural RNAs that promote cleavage and ligation of phosphodiester bonds to conduct RNA splicing. As naturally occurring group I ribozymes are constitutively active in most cases, modular engineering is required to apply them to control gene expression. We first introduce exceptional examples of modified splicing reactions of group I ribozymes. We then summarize strategies to engineer group I ribozymes to develop genetic modular tools and also their application to gene expression control.

AB - Group I ribozymes (RNA enzymes) constitute a class of structural RNAs that promote cleavage and ligation of phosphodiester bonds to conduct RNA splicing. As naturally occurring group I ribozymes are constitutively active in most cases, modular engineering is required to apply them to control gene expression. We first introduce exceptional examples of modified splicing reactions of group I ribozymes. We then summarize strategies to engineer group I ribozymes to develop genetic modular tools and also their application to gene expression control.

KW - Alternative splicing

KW - Aptamer

KW - Group I intron

KW - RNA structure

KW - Ribozyme

KW - Self-splicing

KW - Trans-splicing

UR - http://www.scopus.com/inward/record.url?scp=85053979988&partnerID=8YFLogxK

U2 - 10.1007/978-981-10-8372-3_13

DO - 10.1007/978-981-10-8372-3_13

M3 - 章

AN - SCOPUS:85053979988

SN - 9789811083716

SP - 203

EP - 220

BT - Applied RNA Bioscience

PB - Springer Singapore

ER -

Engineered group i ribozymes as RNA-based modular tools to control gene expression

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this