Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain

Tomoya Nakamachi; Hirokazu Ohtaki; Sachiko Yofu; Kenji Dohi; Jun Watanabe; Hiroyoshi Mori; Atsushi Sato; Hitoshi Hashimoto; Norihito Shintani; Akemichi Baba; Seiji Shioda

doi:10.1007/978-3-211-98811-4_6

Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain

Tomoya Nakamachi, Hirokazu Ohtaki, Sachiko Yofu, Kenji Dohi, Jun Watanabe, Hiroyoshi Mori, Atsushi Sato, Hitoshi Hashimoto, Norihito Shintani, Akemichi Baba, Seiji Shioda^*

^*Corresponding author for this work

Department of Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

35 Scopus citations

Abstract

Pituitary adenylate cyclase activating polypeptide is a pleiotropic neuropeptide. We previously showed that heterozygous PACAP gene knockout (PACAP^+/-) mice had larger infarct volumes and worse neurological scores after middle cerebral artery occlusion (MCAO). However, the relationship between endogenous PACAP levels and edema in the ischemic brain has not yet been evaluated. In this study, the formation of edema in the ischemic brain as well as cerebral blood flow was compared between PACAP^+/- and wild-type (PACAP^+/+) mice. The amount of brain edema was calculated by subtracting the contralateral volume from the ipsilateral volume 24 h after permanent MCAO. PACAP^+/- mice showed significantly greater brain edema than PACAP^+/+ mice. To investigate the effects of endogenous PACAP on blood flow during ischemia, cerebral blood flow in the ipsilateral and the contralateral cortices was compared between PACAP^+/- and PACAP^+/+ mice for 25 min after ischemia. With a two-dimensional laser Doppler perfusion imaging system, the blood flow in the ipsilateral and contralateral cortices was shown to be similar in PACAP^+/- and PACAP^+/+ mice during ischemia. These results suggest that endogenous PACAP suppresses the formation of edema in the ischemic brain.

Original language	English
Title of host publication	Brain Edema XIV
Publisher	Springer-Verlag Wien
Pages	43-46
Number of pages	4
Edition	106
ISBN (Print)	9783211987582
DOIs	https://doi.org/10.1007/978-3-211-98811-4_6
State	Published - 2009

Publication series

Name	Acta Neurochirurgica, Supplementum
Number	106
ISSN (Print)	0065-1419

Keywords

PACAP
brain edema
cerebral blood flow
knockout
mouse
neural peptides
stroke

ASJC Scopus subject areas

Surgery
Clinical Neurology

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10.1007/978-3-211-98811-4_6

Cite this

Nakamachi, T., Ohtaki, H., Yofu, S., Dohi, K., Watanabe, J., Mori, H., Sato, A., Hashimoto, H., Shintani, N., Baba, A., & Shioda, S. (2009). Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain. In Brain Edema XIV (106 ed., pp. 43-46). (Acta Neurochirurgica, Supplementum; No. 106). Springer-Verlag Wien. https://doi.org/10.1007/978-3-211-98811-4_6

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title = "Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain",

abstract = "Pituitary adenylate cyclase activating polypeptide is a pleiotropic neuropeptide. We previously showed that heterozygous PACAP gene knockout (PACAP+/-) mice had larger infarct volumes and worse neurological scores after middle cerebral artery occlusion (MCAO). However, the relationship between endogenous PACAP levels and edema in the ischemic brain has not yet been evaluated. In this study, the formation of edema in the ischemic brain as well as cerebral blood flow was compared between PACAP+/- and wild-type (PACAP+/+) mice. The amount of brain edema was calculated by subtracting the contralateral volume from the ipsilateral volume 24 h after permanent MCAO. PACAP+/- mice showed significantly greater brain edema than PACAP+/+ mice. To investigate the effects of endogenous PACAP on blood flow during ischemia, cerebral blood flow in the ipsilateral and the contralateral cortices was compared between PACAP+/- and PACAP+/+ mice for 25 min after ischemia. With a two-dimensional laser Doppler perfusion imaging system, the blood flow in the ipsilateral and contralateral cortices was shown to be similar in PACAP+/- and PACAP+/+ mice during ischemia. These results suggest that endogenous PACAP suppresses the formation of edema in the ischemic brain.",

keywords = "PACAP, brain edema, cerebral blood flow, knockout, mouse, neural peptides, stroke",

author = "Tomoya Nakamachi and Hirokazu Ohtaki and Sachiko Yofu and Kenji Dohi and Jun Watanabe and Hiroyoshi Mori and Atsushi Sato and Hitoshi Hashimoto and Norihito Shintani and Akemichi Baba and Seiji Shioda",

note = "Funding Information: We thank Dr. Jerome L. Maderdrut (Tulane University) for his editorial assistance. This work was supported in part by a Showa University Grant-in-Aid for Innovative Collaborative Research Projects and a Special Research Grant-in-Aid for Development of Characteristic Education from the Ministry of Education, Culture, Sports, Science, and Technology (T.N. and S.S.) and Research on Health Sciences focusing on Drug Innovation from The Japan Health Sciences Foundation (S.S).",

year = "2009",

doi = "10.1007/978-3-211-98811-4_6",

language = "英語",

isbn = "9783211987582",

series = "Acta Neurochirurgica, Supplementum",

publisher = "Springer-Verlag Wien",

number = "106",

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Nakamachi, T, Ohtaki, H, Yofu, S, Dohi, K, Watanabe, J, Mori, H, Sato, A, Hashimoto, H, Shintani, N, Baba, A & Shioda, S 2009, Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain. in Brain Edema XIV. 106 edn, Acta Neurochirurgica, Supplementum, no. 106, Springer-Verlag Wien, pp. 43-46. https://doi.org/10.1007/978-3-211-98811-4_6

Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain. / Nakamachi, Tomoya; Ohtaki, Hirokazu; Yofu, Sachiko et al.
Brain Edema XIV. 106. ed. Springer-Verlag Wien, 2009. p. 43-46 (Acta Neurochirurgica, Supplementum; No. 106).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain

AU - Nakamachi, Tomoya

AU - Ohtaki, Hirokazu

AU - Yofu, Sachiko

AU - Dohi, Kenji

AU - Watanabe, Jun

AU - Mori, Hiroyoshi

AU - Sato, Atsushi

AU - Hashimoto, Hitoshi

AU - Shintani, Norihito

AU - Baba, Akemichi

AU - Shioda, Seiji

N1 - Funding Information: We thank Dr. Jerome L. Maderdrut (Tulane University) for his editorial assistance. This work was supported in part by a Showa University Grant-in-Aid for Innovative Collaborative Research Projects and a Special Research Grant-in-Aid for Development of Characteristic Education from the Ministry of Education, Culture, Sports, Science, and Technology (T.N. and S.S.) and Research on Health Sciences focusing on Drug Innovation from The Japan Health Sciences Foundation (S.S).

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N2 - Pituitary adenylate cyclase activating polypeptide is a pleiotropic neuropeptide. We previously showed that heterozygous PACAP gene knockout (PACAP+/-) mice had larger infarct volumes and worse neurological scores after middle cerebral artery occlusion (MCAO). However, the relationship between endogenous PACAP levels and edema in the ischemic brain has not yet been evaluated. In this study, the formation of edema in the ischemic brain as well as cerebral blood flow was compared between PACAP+/- and wild-type (PACAP+/+) mice. The amount of brain edema was calculated by subtracting the contralateral volume from the ipsilateral volume 24 h after permanent MCAO. PACAP+/- mice showed significantly greater brain edema than PACAP+/+ mice. To investigate the effects of endogenous PACAP on blood flow during ischemia, cerebral blood flow in the ipsilateral and the contralateral cortices was compared between PACAP+/- and PACAP+/+ mice for 25 min after ischemia. With a two-dimensional laser Doppler perfusion imaging system, the blood flow in the ipsilateral and contralateral cortices was shown to be similar in PACAP+/- and PACAP+/+ mice during ischemia. These results suggest that endogenous PACAP suppresses the formation of edema in the ischemic brain.

AB - Pituitary adenylate cyclase activating polypeptide is a pleiotropic neuropeptide. We previously showed that heterozygous PACAP gene knockout (PACAP+/-) mice had larger infarct volumes and worse neurological scores after middle cerebral artery occlusion (MCAO). However, the relationship between endogenous PACAP levels and edema in the ischemic brain has not yet been evaluated. In this study, the formation of edema in the ischemic brain as well as cerebral blood flow was compared between PACAP+/- and wild-type (PACAP+/+) mice. The amount of brain edema was calculated by subtracting the contralateral volume from the ipsilateral volume 24 h after permanent MCAO. PACAP+/- mice showed significantly greater brain edema than PACAP+/+ mice. To investigate the effects of endogenous PACAP on blood flow during ischemia, cerebral blood flow in the ipsilateral and the contralateral cortices was compared between PACAP+/- and PACAP+/+ mice for 25 min after ischemia. With a two-dimensional laser Doppler perfusion imaging system, the blood flow in the ipsilateral and contralateral cortices was shown to be similar in PACAP+/- and PACAP+/+ mice during ischemia. These results suggest that endogenous PACAP suppresses the formation of edema in the ischemic brain.

KW - PACAP

KW - brain edema

KW - cerebral blood flow

KW - knockout

KW - mouse

KW - neural peptides

KW - stroke

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M3 - 会議への寄与

C2 - 19812918

AN - SCOPUS:78649339577

SN - 9783211987582

T3 - Acta Neurochirurgica, Supplementum

SP - 43

EP - 46

BT - Brain Edema XIV

PB - Springer-Verlag Wien

ER -

Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain

Abstract

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Keywords

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