Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

Hiroshi Ageta; Akihiko Kato; Yugo Fukazawa; Kaoru Inokuchi; Hiroyuki Sugiyama

doi:10.1016/S0169-328X(01)00303-5

Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

Hiroshi Ageta, Akihiko Kato, Yugo Fukazawa, Kaoru Inokuchi, Hiroyuki Sugiyama^*

^*Corresponding author for this work

Biochemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.

Original language	English
Pages (from-to)	186-189
Number of pages	4
Journal	Molecular Brain Research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(01)00303-5
State	Published - 2001/12/30

Keywords

Lactacystin
MG132
Proteasome
Synaptic placity
Vesl-1S/Homer-1a

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(01)00303-5

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title = "Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins",

abstract = "The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.",

keywords = "Lactacystin, MG132, Proteasome, Synaptic placity, Vesl-1S/Homer-1a",

author = "Hiroshi Ageta and Akihiko Kato and Yugo Fukazawa and Kaoru Inokuchi and Hiroyuki Sugiyama",

year = "2001",

month = dec,

day = "30",

doi = "10.1016/S0169-328X(01)00303-5",

language = "英語",

volume = "97",

pages = "186--189",

journal = "Molecular Brain Research",

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TY - JOUR

T1 - Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

AU - Ageta, Hiroshi

AU - Kato, Akihiko

AU - Fukazawa, Yugo

AU - Inokuchi, Kaoru

AU - Sugiyama, Hiroyuki

PY - 2001/12/30

Y1 - 2001/12/30

N2 - The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.

AB - The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.

KW - Lactacystin

KW - MG132

KW - Proteasome

KW - Synaptic placity

KW - Vesl-1S/Homer-1a

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U2 - 10.1016/S0169-328X(01)00303-5

DO - 10.1016/S0169-328X(01)00303-5

M3 - 学術論文

C2 - 11750075

AN - SCOPUS:0035977412

SN - 0169-328X

VL - 97

SP - 186

EP - 189

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -

Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

Abstract

Keywords

ASJC Scopus subject areas

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