Cloning and characterization of rat casein kinase 1ε

Atsuko Takano; Kimiko Shimizu; Shuichi Kani; Ruud M. Buijs; Masato Okada; Katsuya Nagai

doi:10.1016/S0014-5793(00)01755-5

Cloning and characterization of rat casein kinase 1ε

Atsuko Takano^*, Kimiko Shimizu, Shuichi Kani, Ruud M. Buijs, Masato Okada, Katsuya Nagai

^*Corresponding author for this work

International IR / Security Export Control Division, Organization for International Education and Exchange

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ε (CK1ε) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ε and characterization of the protein products. The rCK1ε is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. Copyright (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	FEBS Letters
Volume	477
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(00)01755-5
State	Published - 2000/07/14

Keywords

Circadian rhythm
Differential display
Nuclear translocation
Per
Phosphorylation
Suprachiasmatic nucleus

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)01755-5

Cite this

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title = "Cloning and characterization of rat casein kinase 1ε",

abstract = "Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ε (CK1ε) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ε and characterization of the protein products. The rCK1ε is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. Copyright (C) 2000 Federation of European Biochemical Societies.",

keywords = "Circadian rhythm, Differential display, Nuclear translocation, Per, Phosphorylation, Suprachiasmatic nucleus",

author = "Atsuko Takano and Kimiko Shimizu and Shuichi Kani and Buijs, {Ruud M.} and Masato Okada and Katsuya Nagai",

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doi = "10.1016/S0014-5793(00)01755-5",

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T1 - Cloning and characterization of rat casein kinase 1ε

AU - Takano, Atsuko

AU - Shimizu, Kimiko

AU - Kani, Shuichi

AU - Buijs, Ruud M.

AU - Okada, Masato

AU - Nagai, Katsuya

PY - 2000/7/14

Y1 - 2000/7/14

N2 - Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ε (CK1ε) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ε and characterization of the protein products. The rCK1ε is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. Copyright (C) 2000 Federation of European Biochemical Societies.

AB - Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1ε (CK1ε) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1ε and characterization of the protein products. The rCK1ε is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. Copyright (C) 2000 Federation of European Biochemical Societies.

KW - Circadian rhythm

KW - Differential display

KW - Nuclear translocation

KW - Per

KW - Phosphorylation

KW - Suprachiasmatic nucleus

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SN - 0014-5793

VL - 477

SP - 106

EP - 112

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Cloning and characterization of rat casein kinase 1ε

Abstract

Keywords

ASJC Scopus subject areas

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