TY - JOUR
T1 - Carboxymethylbetaine copolymer layer covalently fixed to a glass substrate
AU - Suzuki, Hisatomo
AU - Li, Lifu
AU - Nakaji-Hirabayashi, Tadashi
AU - Kitano, Hiromi
AU - Ohno, Kohji
AU - Matsuoka, Kazuyoshi
AU - Saruwatari, Yoshiyuki
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research ( 22350101 ) from the Japan Society for the Promotion of Science (JSPS) , and a Grant-in-Aid ( 20106007 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Tokyo, Japan . The authors are indebted to Osaka Organic Chemical Industry for the continuous support to pursue this work. The authors are also grateful to Professor T. Tsujii, Kyoto University, for allowing us to use the ellipsometer. The authors wish to thank Mr. Sho Saito of this department for his technical assistance.
PY - 2012/6/1
Y1 - 2012/6/1
N2 - A random copolymer of zwitterionic monomer, carboxymethylbetaine (CMB), and 3-methacryloyloxypropyl trimethoxysilane was prepared in ethanol using 2,2'-azobisisobutyronitrile as initiator. The incubation of ethanol solution of the copolymer with a glass plate gave a layer of the copolymer with a thickness of about 2-3. nm. The copolymer-modified glass substrate became highly hydrophilic upon immersion in water, and showed a resistance against non-specific adsorption of proteins, and the degree of resistance increased with the content of CMB residues in the copolymer and leveled off. The adhesion of various cells to the glass substrate was also strongly suppressed by the surface modification with the copolymer layer. Further introduction of PolyCMB graft chains on the surface of the layer enhanced the suppression of cell adhesion due to the steric hindrance for the cells to approach the layer. The usefulness of the combination of zwitterionic polymer layer and graft chains to afford anti-biofouling properties to a solid surface of metal oxides was shown.
AB - A random copolymer of zwitterionic monomer, carboxymethylbetaine (CMB), and 3-methacryloyloxypropyl trimethoxysilane was prepared in ethanol using 2,2'-azobisisobutyronitrile as initiator. The incubation of ethanol solution of the copolymer with a glass plate gave a layer of the copolymer with a thickness of about 2-3. nm. The copolymer-modified glass substrate became highly hydrophilic upon immersion in water, and showed a resistance against non-specific adsorption of proteins, and the degree of resistance increased with the content of CMB residues in the copolymer and leveled off. The adhesion of various cells to the glass substrate was also strongly suppressed by the surface modification with the copolymer layer. Further introduction of PolyCMB graft chains on the surface of the layer enhanced the suppression of cell adhesion due to the steric hindrance for the cells to approach the layer. The usefulness of the combination of zwitterionic polymer layer and graft chains to afford anti-biofouling properties to a solid surface of metal oxides was shown.
KW - Adhesion of cells
KW - Adsorption of proteins
KW - Anti-biofouling
KW - Graft of zwitterionic polymer
KW - Zwitterionic copolymer layer
UR - http://www.scopus.com/inward/record.url?scp=84862823326&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2012.01.038
DO - 10.1016/j.colsurfb.2012.01.038
M3 - 学術論文
C2 - 22348985
AN - SCOPUS:84862823326
SN - 0927-7765
VL - 94
SP - 107
EP - 113
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -