TY - JOUR
T1 - Benzophenone synthase from Garcinia mangostana L. pericarps
AU - Nualkaew, Natsajee
AU - Morita, Hiroyuki
AU - Shimokawa, Yoshihiko
AU - Kinjo, Keishi
AU - Kushiro, Tetsuo
AU - De-Eknamkul, Wanchai
AU - Ebizuka, Yutaka
AU - Abe, Ikuro
N1 - Funding Information:
We thank Mr. Anuwatchakit Klamrak for assistance. NN was supported by The National Research Council of Thailand – Japan Society for the Promotion of Science (NRCT-JSPS) exchange program and Khon-Kaen University, Thailand. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (to I.A. and H.M.). Y.S. is a recipient of the JSPS Fellowship for Young Scientists.
PY - 2012/5
Y1 - 2012/5
N2 - The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.
AB - The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.
KW - Benzophenone synthase
KW - Clusiaceae
KW - Garcinia mangostana
KW - Polyketide synthase
KW - Xanthones
UR - http://www.scopus.com/inward/record.url?scp=84859158491&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2012.02.002
DO - 10.1016/j.phytochem.2012.02.002
M3 - 学術論文
C2 - 22390826
AN - SCOPUS:84859158491
SN - 0031-9422
VL - 77
SP - 60
EP - 69
JO - Phytochemistry
JF - Phytochemistry
ER -