Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2

Yoshitomo Morinaga; Hiroshi Yamada; Yoshihiro Yoshida; Hitoshi Kawasuji; Yoshihiro Yamamoto

doi:10.1016/j.jiac.2022.10.004

Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2

Yoshitomo Morinaga^*, Hiroshi Yamada, Yoshihiro Yoshida, Hitoshi Kawasuji, Yoshihiro Yamamoto

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Introduction: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. Methods: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow®, ESPLINE® SARS-CoV-2, QuickNavi™ COVID19 Ag, ImmunoAce® SARS-CoV-2, Panbio™ COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow® against the Omicron variants was compared with that against the wild-type using recombinant proteins. Results: ImmunoArrow® and ESPLINE® showed the lowest LoD. The time to positive results of all tests except for ESPLINE® was within 200 s in the evaluation at high dose of antigens (2.5 × 10⁵ TCID₅₀/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 10⁴ TCID₅₀/mL). The LoD of ImmunoArrow® against the Omicron variants was the same concentration against the wild-type antigen. Conclusions: ImmunoArrow® detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity.

Original language	English
Pages (from-to)	131-135
Number of pages	5
Journal	Journal of Infection and Chemotherapy
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/j.jiac.2022.10.004
State	Published - 2023/02

Keywords

Antigen tests
Immunochromatography
Lateral flow tests
Omicron
Point-of-care testing

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

Access to Document

10.1016/j.jiac.2022.10.004

Cite this

@article{3b06d9c5777440f48f17e6db309e6b41,

title = "Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2",

abstract = "Introduction: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. Methods: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow{\textregistered}, ESPLINE{\textregistered} SARS-CoV-2, QuickNavi{\texttrademark} COVID19 Ag, ImmunoAce{\textregistered} SARS-CoV-2, Panbio{\texttrademark} COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow{\textregistered} against the Omicron variants was compared with that against the wild-type using recombinant proteins. Results: ImmunoArrow{\textregistered} and ESPLINE{\textregistered} showed the lowest LoD. The time to positive results of all tests except for ESPLINE{\textregistered} was within 200 s in the evaluation at high dose of antigens (2.5 × 105 TCID50/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 104 TCID50/mL). The LoD of ImmunoArrow{\textregistered} against the Omicron variants was the same concentration against the wild-type antigen. Conclusions: ImmunoArrow{\textregistered} detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity.",

keywords = "Antigen tests, Immunochromatography, Lateral flow tests, Omicron, Point-of-care testing",

author = "Yoshitomo Morinaga and Hiroshi Yamada and Yoshihiro Yoshida and Hitoshi Kawasuji and Yoshihiro Yamamoto",

note = "Publisher Copyright: {\textcopyright} 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases",

year = "2023",

month = feb,

doi = "10.1016/j.jiac.2022.10.004",

language = "英語",

volume = "29",

pages = "131--135",

journal = "Journal of Infection and Chemotherapy",

issn = "1341-321X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2

AU - Morinaga, Yoshitomo

AU - Yamada, Hiroshi

AU - Yoshida, Yoshihiro

AU - Kawasuji, Hitoshi

AU - Yamamoto, Yoshihiro

PY - 2023/2

Y1 - 2023/2

N2 - Introduction: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. Methods: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow®, ESPLINE® SARS-CoV-2, QuickNavi™ COVID19 Ag, ImmunoAce® SARS-CoV-2, Panbio™ COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow® against the Omicron variants was compared with that against the wild-type using recombinant proteins. Results: ImmunoArrow® and ESPLINE® showed the lowest LoD. The time to positive results of all tests except for ESPLINE® was within 200 s in the evaluation at high dose of antigens (2.5 × 105 TCID50/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 104 TCID50/mL). The LoD of ImmunoArrow® against the Omicron variants was the same concentration against the wild-type antigen. Conclusions: ImmunoArrow® detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity.

AB - Introduction: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. Methods: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow®, ESPLINE® SARS-CoV-2, QuickNavi™ COVID19 Ag, ImmunoAce® SARS-CoV-2, Panbio™ COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow® against the Omicron variants was compared with that against the wild-type using recombinant proteins. Results: ImmunoArrow® and ESPLINE® showed the lowest LoD. The time to positive results of all tests except for ESPLINE® was within 200 s in the evaluation at high dose of antigens (2.5 × 105 TCID50/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 104 TCID50/mL). The LoD of ImmunoArrow® against the Omicron variants was the same concentration against the wild-type antigen. Conclusions: ImmunoArrow® detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity.

KW - Antigen tests

KW - Immunochromatography

KW - Lateral flow tests

KW - Omicron

KW - Point-of-care testing

UR - http://www.scopus.com/inward/record.url?scp=85141868011&partnerID=8YFLogxK

U2 - 10.1016/j.jiac.2022.10.004

DO - 10.1016/j.jiac.2022.10.004

M3 - 学術論文

C2 - 36265820

AN - SCOPUS:85141868011

SN - 1341-321X

VL - 29

SP - 131

EP - 135

JO - Journal of Infection and Chemotherapy

JF - Journal of Infection and Chemotherapy

IS - 2

ER -

Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this