Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Takashi Okitsu; Take Matsuyama; Takahiro Yamashit; Toru Ishizuk; Hiromu Yawo; Yasushi Imamoto; Yoshinori Shichid; Akimori Wada

doi:10.1248/cpb.c17-00054

Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Takashi Okitsu, Take Matsuyama, Takahiro Yamashit, Toru Ishizuk, Hiromu Yawo, Yasushi Imamoto, Yoshinori Shichid, Akimori Wada^*

^*Corresponding author for this work

Synthetic and Biomolecular Organic Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

Original language	English
Pages (from-to)	356-358
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	65
Issue number	4
DOIs	https://doi.org/10.1248/cpb.c17-00054
State	Published - 2017

Keywords

Channelrhodopsin
Chromophore
Neuroscience
Optogenetics
Red-shift

ASJC Scopus subject areas

General Chemistry
Drug Discovery

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10.1248/cpb.c17-00054

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title = "Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin",

abstract = "Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.",

keywords = "Channelrhodopsin, Chromophore, Neuroscience, Optogenetics, Red-shift",

author = "Takashi Okitsu and Take Matsuyama and Takahiro Yamashit and Toru Ishizuk and Hiromu Yawo and Yasushi Imamoto and Yoshinori Shichid and Akimori Wada",

note = "Publisher Copyright: {\textcopyright} 2017 The Pharmaceutical Society of Japan.",

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doi = "10.1248/cpb.c17-00054",

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T2 - Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

AU - Okitsu, Takashi

AU - Matsuyama, Take

AU - Yamashit, Takahiro

AU - Ishizuk, Toru

AU - Yawo, Hiromu

AU - Imamoto, Yasushi

AU - Shichid, Yoshinori

AU - Wada, Akimori

PY - 2017

Y1 - 2017

N2 - Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

AB - Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30 nm toward longer wavelengths than that of C1C2-ChR regenerated with alltrans- retinal.

KW - Channelrhodopsin

KW - Chromophore

KW - Neuroscience

KW - Optogenetics

KW - Red-shift

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DO - 10.1248/cpb.c17-00054

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AN - SCOPUS:85017299853

SN - 0009-2363

VL - 65

SP - 356

EP - 358

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

IS - 4

ER -

Alternative formation of red-shifted channelrhodopsins: Noncovalent incorporation with retinal-based enamine-type schiff bases and mutated channelopsin

Abstract

Keywords

ASJC Scopus subject areas

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