Abstract
Objective: We investigated α-7 nAchR expression in human peritoneal macrophages and examined whether activation of nAchR might be a new therapy for endometriosis. Materials and methods: Human peritoneal fluid mononuclear cells (PFMC) were stimulated with lipopolysaccharide (LPS) in the presence of α-7 nAChR agonists. In a murine endometriosis model, α-7 nAChR modulators were administered. Results: Human PFMC expressed α-7 nAChR at the mRNA and protein levels. Activation of α-7 nAChR with its agonists led to significant (P<.01) suppression of LPS-induced interleukin (IL) -1β expression. In a murine endometriosis model, one week after inoculation of endometrium to the peritoneal cavity, α-7 nAChR agonist significantly suppressed the expression of IL-1β mRNA (P<.01), which was negated when α-7 nAChR antagonist was administered simultaneously. α-7 nAChR agonist significantly suppressed the formation of endometriotic lesions, which was reversed with α-7 nAChR antagonist. Conclusion: Activation of nAChR might be a new candidate for treatment of endometriosis.
Original language | English |
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Pages (from-to) | 491-498 |
Number of pages | 8 |
Journal | American Journal of Reproductive Immunology |
Volume | 76 |
Issue number | 6 |
DOIs | |
State | Published - 2016/12/01 |
Keywords
- endometriosis
- inflammation
- nicotine
- α-7 nAChR
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Reproductive Medicine
- Obstetrics and Gynecology