A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

Fumio Harada; Yutaka Hirose; Motohiro Tanaka; Takuma Sasaki; Hiroyuki Kamiya; Kazunobu Miura

doi:10.1111/j.1349-7006.1990.tb02555.x

A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

Fumio Harada^*, Yutaka Hirose, Motohiro Tanaka, Takuma Sasaki, Hiroyuki Kamiya, Kazunobu Miura

^*Corresponding author for this work

Gene Regulation

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A⁺ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA^Pro‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.

Original language	English
Pages (from-to)	232-237
Number of pages	6
Journal	Japanese Journal of Cancer Research
Volume	81
Issue number	3
DOIs	https://doi.org/10.1111/j.1349-7006.1990.tb02555.x
State	Published - 1990/03

Keywords

Primer binding site
Primer tRNA
Retrovirus
Viral RNA
Xenotropic murine leukemia virus

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1111/j.1349-7006.1990.tb02555.x

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title = "A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells",

abstract = "A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNAPro‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.",

keywords = "Primer binding site, Primer tRNA, Retrovirus, Viral RNA, Xenotropic murine leukemia virus",

author = "Fumio Harada and Yutaka Hirose and Motohiro Tanaka and Takuma Sasaki and Hiroyuki Kamiya and Kazunobu Miura",

year = "1990",

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doi = "10.1111/j.1349-7006.1990.tb02555.x",

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AU - Harada, Fumio

AU - Hirose, Yutaka

AU - Tanaka, Motohiro

AU - Sasaki, Takuma

AU - Kamiya, Hiroyuki

AU - Miura, Kazunobu

PY - 1990/3

Y1 - 1990/3

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AB - A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNAPro‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.

KW - Primer binding site

KW - Primer tRNA

KW - Retrovirus

KW - Viral RNA

KW - Xenotropic murine leukemia virus

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M3 - 学術論文

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AN - SCOPUS:0025216533

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JO - Japanese Journal of Cancer Research

JF - Japanese Journal of Cancer Research

IS - 3

ER -

A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

Abstract

Keywords

ASJC Scopus subject areas

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