A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination

Hideki Niimi*, Masashi Mori, Homare Tabata, Hiroshi Minami, Tomohiro Ueno, Shirou Hayashi, Isao Kitajima

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.

Original languageEnglish
Pages (from-to)3316-3320
Number of pages5
JournalJournal of Clinical Microbiology
Volume49
Issue number9
DOIs
StatePublished - 2011/09

ASJC Scopus subject areas

  • Microbiology (medical)

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