A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

Makiko Nishi-Tatsumi; Naoya Yahagi; Yoshinori Takeuchi; Naoki Toya; Ayako Takarada; Yuki Murayama; Yuichi Aita; Yoshikazu Sawada; Xiaoying Piao; Yukari Oya; Akito Shikama; Yukari Masuda; Midori Kubota; Yoshihiko Izumida; Takashi Matsuzaka; Yoshimi Nakagawa; Motohiro Sekiya; Yoko Iizuka; Yasushi Kawakami; Takashi Kadowaki; Nobuhiro Yamada; Hitoshi Shimano

doi:10.1002/1873-3468.12620

A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

Makiko Nishi-Tatsumi, Naoya Yahagi^*, Yoshinori Takeuchi, Naoki Toya, Ayako Takarada, Yuki Murayama, Yuichi Aita, Yoshikazu Sawada, Xiaoying Piao, Yukari Oya, Akito Shikama, Yukari Masuda, Midori Kubota, Yoshihiko Izumida, Takashi Matsuzaka, Yoshimi Nakagawa, Motohiro Sekiya, Yoko Iizuka, Yasushi Kawakami, Takashi KadowakiNobuhiro Yamada, Hitoshi Shimano

^*Corresponding author for this work

Complex Biosystem Research, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

Original language	English
Pages (from-to)	965-978
Number of pages	14
Journal	FEBS Letters
Volume	591
Issue number	7
DOIs	https://doi.org/10.1002/1873-3468.12620
State	Published - 2017/04/01

Keywords

adipose tissue
fatty acid
transcription

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Nishi-Tatsumi, M., Yahagi, N., Takeuchi, Y., Toya, N., Takarada, A., Murayama, Y., Aita, Y., Sawada, Y., Piao, X., Oya, Y., Shikama, A., Masuda, Y., Kubota, M., Izumida, Y., Matsuzaka, T., Nakagawa, Y., Sekiya, M., Iizuka, Y., Kawakami, Y., ... Shimano, H. (2017). A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes. FEBS Letters, 591(7), 965-978. https://doi.org/10.1002/1873-3468.12620

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abstract = "Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.",

keywords = "adipose tissue, fatty acid, transcription",

author = "Makiko Nishi-Tatsumi and Naoya Yahagi and Yoshinori Takeuchi and Naoki Toya and Ayako Takarada and Yuki Murayama and Yuichi Aita and Yoshikazu Sawada and Xiaoying Piao and Yukari Oya and Akito Shikama and Yukari Masuda and Midori Kubota and Yoshihiko Izumida and Takashi Matsuzaka and Yoshimi Nakagawa and Motohiro Sekiya and Yoko Iizuka and Yasushi Kawakami and Takashi Kadowaki and Nobuhiro Yamada and Hitoshi Shimano",

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doi = "10.1002/1873-3468.12620",

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Nishi-Tatsumi, M, Yahagi, N, Takeuchi, Y, Toya, N, Takarada, A, Murayama, Y, Aita, Y, Sawada, Y, Piao, X, Oya, Y, Shikama, A, Masuda, Y, Kubota, M, Izumida, Y, Matsuzaka, T, Nakagawa, Y, Sekiya, M, Iizuka, Y, Kawakami, Y, Kadowaki, T, Yamada, N & Shimano, H 2017, 'A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes', FEBS Letters, vol. 591, no. 7, pp. 965-978. https://doi.org/10.1002/1873-3468.12620

TY - JOUR

T1 - A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

AU - Nishi-Tatsumi, Makiko

AU - Yahagi, Naoya

AU - Takeuchi, Yoshinori

AU - Toya, Naoki

AU - Takarada, Ayako

AU - Murayama, Yuki

AU - Aita, Yuichi

AU - Sawada, Yoshikazu

AU - Piao, Xiaoying

AU - Oya, Yukari

AU - Shikama, Akito

AU - Masuda, Yukari

AU - Kubota, Midori

AU - Izumida, Yoshihiko

AU - Matsuzaka, Takashi

AU - Nakagawa, Yoshimi

AU - Sekiya, Motohiro

AU - Iizuka, Yoko

AU - Kawakami, Yasushi

AU - Kadowaki, Takashi

AU - Yamada, Nobuhiro

AU - Shimano, Hitoshi

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

AB - Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

KW - adipose tissue

KW - fatty acid

KW - transcription

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JO - FEBS Letters

JF - FEBS Letters

IS - 7

ER -

A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

Abstract

Keywords

ASJC Scopus subject areas

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