A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

Makiko Nishi-Tatsumi; Naoya Yahagi; Yoshinori Takeuchi; Naoki Toya; Ayako Takarada; Yuki Murayama; Yuichi Aita; Yoshikazu Sawada; Xiaoying Piao; Yukari Oya; Akito Shikama; Yukari Masuda; Midori Kubota; Yoshihiko Izumida; Takashi Matsuzaka; Yoshimi Nakagawa; Motohiro Sekiya; Yoko Iizuka; Yasushi Kawakami; Takashi Kadowaki; Nobuhiro Yamada; Hitoshi Shimano

doi:10.1002/1873-3468.12620

A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

Makiko Nishi-Tatsumi, Naoya Yahagi^*, Yoshinori Takeuchi, Naoki Toya, Ayako Takarada, Yuki Murayama, Yuichi Aita, Yoshikazu Sawada, Xiaoying Piao, Yukari Oya, Akito Shikama, Yukari Masuda, Midori Kubota, Yoshihiko Izumida, Takashi Matsuzaka, Yoshimi Nakagawa, Motohiro Sekiya, Yoko Iizuka, Yasushi Kawakami, Takashi KadowakiNobuhiro Yamada, Hitoshi Shimano

^*この論文の責任著者

和漢医薬学総合研究所研究開発部門複雑系解析分野

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

12 被引用数 (Scopus)

抄録

Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

本文言語	英語
ページ（範囲）	965-978
ページ数	14
ジャーナル	FEBS Letters
巻	591
号	7
DOI	https://doi.org/10.1002/1873-3468.12620
出版ステータス	出版済み - 2017/04/01

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

文献へのアクセス

10.1002/1873-3468.12620

引用スタイル

Nishi-Tatsumi, M., Yahagi, N., Takeuchi, Y., Toya, N., Takarada, A., Murayama, Y., Aita, Y., Sawada, Y., Piao, X., Oya, Y., Shikama, A., Masuda, Y., Kubota, M., Izumida, Y., Matsuzaka, T., Nakagawa, Y., Sekiya, M., Iizuka, Y., Kawakami, Y., ... Shimano, H. (2017). A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes. FEBS Letters, 591(7), 965-978. https://doi.org/10.1002/1873-3468.12620

@article{f33e91d7614a440ba7ae5cdfe035600e,

title = "A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes",

abstract = "Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.",

keywords = "adipose tissue, fatty acid, transcription",

author = "Makiko Nishi-Tatsumi and Naoya Yahagi and Yoshinori Takeuchi and Naoki Toya and Ayako Takarada and Yuki Murayama and Yuichi Aita and Yoshikazu Sawada and Xiaoying Piao and Yukari Oya and Akito Shikama and Yukari Masuda and Midori Kubota and Yoshihiko Izumida and Takashi Matsuzaka and Yoshimi Nakagawa and Motohiro Sekiya and Yoko Iizuka and Yasushi Kawakami and Takashi Kadowaki and Nobuhiro Yamada and Hitoshi Shimano",

note = "Publisher Copyright: {\textcopyright} 2017 Federation of European Biochemical Societies",

year = "2017",

month = apr,

day = "1",

doi = "10.1002/1873-3468.12620",

language = "英語",

volume = "591",

pages = "965--978",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "7",

}

Nishi-Tatsumi, M, Yahagi, N, Takeuchi, Y, Toya, N, Takarada, A, Murayama, Y, Aita, Y, Sawada, Y, Piao, X, Oya, Y, Shikama, A, Masuda, Y, Kubota, M, Izumida, Y, Matsuzaka, T, Nakagawa, Y, Sekiya, M, Iizuka, Y, Kawakami, Y, Kadowaki, T, Yamada, N & Shimano, H 2017, 'A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes', FEBS Letters, vol. 591, no. 7, pp. 965-978. https://doi.org/10.1002/1873-3468.12620

TY - JOUR

T1 - A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

AU - Nishi-Tatsumi, Makiko

AU - Yahagi, Naoya

AU - Takeuchi, Yoshinori

AU - Toya, Naoki

AU - Takarada, Ayako

AU - Murayama, Yuki

AU - Aita, Yuichi

AU - Sawada, Yoshikazu

AU - Piao, Xiaoying

AU - Oya, Yukari

AU - Shikama, Akito

AU - Masuda, Yukari

AU - Kubota, Midori

AU - Izumida, Yoshihiko

AU - Matsuzaka, Takashi

AU - Nakagawa, Yoshimi

AU - Sekiya, Motohiro

AU - Iizuka, Yoko

AU - Kawakami, Yasushi

AU - Kadowaki, Takashi

AU - Yamada, Nobuhiro

AU - Shimano, Hitoshi

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

AB - Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using the in vivo Ad-luc analytical system, we identified the inverted CCAAT element (ICE) around −100 nucleotides in the Fasn promoter as a critical cis-element for the refeeding response in adipocytes. Electrophoretic mobility shift assays and chromatin immunoprecipitation show that nuclear factor Y (NF-Y) binds to ICE specifically in refeeding states. Notably, the NF-Y binding to ICE is differently regulated between adipocytes and hepatocytes. These findings provide insights into the specific mechanisms controlling energy metabolism in adipocytes.

KW - adipose tissue

KW - fatty acid

KW - transcription

UR - http://www.scopus.com/inward/record.url?scp=85016462460&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.12620

DO - 10.1002/1873-3468.12620

M3 - 学術論文

C2 - 28281280

AN - SCOPUS:85016462460

SN - 0014-5793

VL - 591

SP - 965

EP - 978

JO - FEBS Letters

JF - FEBS Letters

IS - 7

ER -

A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes

抄録

ASJC Scopus 主題領域

文献へのアクセス

フィンガープリント

引用スタイル