αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets

Yukiko Enomoto; Seiji Adachi; Rie Matsushima-Nishiwaki; Masayuki Niwa; Haruhiko Tokuda; Shigeru Akamatsu; Tomoaki Doi; Hisaaki Kato; Shinichi Yoshimura; Shinji Ogura; Toru Iwama; Osamu Kozawa

doi:10.1016/j.febslet.2009.06.036

αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets

Yukiko Enomoto, Seiji Adachi, Rie Matsushima-Nishiwaki, Masayuki Niwa, Haruhiko Tokuda, Shigeru Akamatsu, Tomoaki Doi, Hisaaki Kato, Shinichi Yoshimura, Shinji Ogura, Toru Iwama, Osamu Kozawa^*

^*Corresponding author for this work

Emergency Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

αB-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of αB-crystallin is not clarified. In this study, we investigated the effect of αB-crystallin on platelet granule secretion. αB-crystallin attenuated the adenosine diphosphate (ADP)-induced phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK) and p38 MAPK. The ADP-stimulated HSP27 phosphorylation was markedly reduced by αB-crystallin. αB-crystallin significantly suppressed the ADP-induced secretions of both platelet-derived growth factor (PDGF)-AB and serotonin. Therefore, our results strongly suggest that αB-crystallin extracellularly suppresses platelet granule secretion by inhibition of HSP27 phosphorylation via p44/p42 MAPK and p38 MAPK.

Original language	English
Pages (from-to)	2464-2468
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2009.06.036
State	Published - 2009/08/06

Keywords

Adenosine diphosphate
Heat shock protein
Platelet
Platelet-derived growth factor-AB
Serotonin
αB-crystallin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets",

abstract = "αB-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of αB-crystallin is not clarified. In this study, we investigated the effect of αB-crystallin on platelet granule secretion. αB-crystallin attenuated the adenosine diphosphate (ADP)-induced phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK) and p38 MAPK. The ADP-stimulated HSP27 phosphorylation was markedly reduced by αB-crystallin. αB-crystallin significantly suppressed the ADP-induced secretions of both platelet-derived growth factor (PDGF)-AB and serotonin. Therefore, our results strongly suggest that αB-crystallin extracellularly suppresses platelet granule secretion by inhibition of HSP27 phosphorylation via p44/p42 MAPK and p38 MAPK.",

keywords = "Adenosine diphosphate, Heat shock protein, Platelet, Platelet-derived growth factor-AB, Serotonin, αB-crystallin",

author = "Yukiko Enomoto and Seiji Adachi and Rie Matsushima-Nishiwaki and Masayuki Niwa and Haruhiko Tokuda and Shigeru Akamatsu and Tomoaki Doi and Hisaaki Kato and Shinichi Yoshimura and Shinji Ogura and Toru Iwama and Osamu Kozawa",

note = "Funding Information: We are very grateful to Yoko Kawamura for her skillful technical assistance. This investigation was supported in part by Grants-in-Aid for Scientific Research (20590565 and 20591825) for the Ministry of Education, Science, Sports and Culture of Japan, the Research Grants for Longevity Sciences (17A-3), Research on Proteomics and Research on Longevity Sciences from the Ministry of Health, Labour and Welfare of Japan.",

year = "2009",

month = aug,

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doi = "10.1016/j.febslet.2009.06.036",

language = "英語",

volume = "583",

pages = "2464--2468",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "15",

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TY - JOUR

T1 - αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets

AU - Enomoto, Yukiko

AU - Adachi, Seiji

AU - Matsushima-Nishiwaki, Rie

AU - Niwa, Masayuki

AU - Tokuda, Haruhiko

AU - Akamatsu, Shigeru

AU - Doi, Tomoaki

AU - Kato, Hisaaki

AU - Yoshimura, Shinichi

AU - Ogura, Shinji

AU - Iwama, Toru

AU - Kozawa, Osamu

N1 - Funding Information: We are very grateful to Yoko Kawamura for her skillful technical assistance. This investigation was supported in part by Grants-in-Aid for Scientific Research (20590565 and 20591825) for the Ministry of Education, Science, Sports and Culture of Japan, the Research Grants for Longevity Sciences (17A-3), Research on Proteomics and Research on Longevity Sciences from the Ministry of Health, Labour and Welfare of Japan.

PY - 2009/8/6

Y1 - 2009/8/6

N2 - αB-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of αB-crystallin is not clarified. In this study, we investigated the effect of αB-crystallin on platelet granule secretion. αB-crystallin attenuated the adenosine diphosphate (ADP)-induced phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK) and p38 MAPK. The ADP-stimulated HSP27 phosphorylation was markedly reduced by αB-crystallin. αB-crystallin significantly suppressed the ADP-induced secretions of both platelet-derived growth factor (PDGF)-AB and serotonin. Therefore, our results strongly suggest that αB-crystallin extracellularly suppresses platelet granule secretion by inhibition of HSP27 phosphorylation via p44/p42 MAPK and p38 MAPK.

AB - αB-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of αB-crystallin is not clarified. In this study, we investigated the effect of αB-crystallin on platelet granule secretion. αB-crystallin attenuated the adenosine diphosphate (ADP)-induced phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK) and p38 MAPK. The ADP-stimulated HSP27 phosphorylation was markedly reduced by αB-crystallin. αB-crystallin significantly suppressed the ADP-induced secretions of both platelet-derived growth factor (PDGF)-AB and serotonin. Therefore, our results strongly suggest that αB-crystallin extracellularly suppresses platelet granule secretion by inhibition of HSP27 phosphorylation via p44/p42 MAPK and p38 MAPK.

KW - Adenosine diphosphate

KW - Heat shock protein

KW - Platelet

KW - Platelet-derived growth factor-AB

KW - Serotonin

KW - αB-crystallin

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U2 - 10.1016/j.febslet.2009.06.036

DO - 10.1016/j.febslet.2009.06.036

M3 - 学術論文

C2 - 19559024

AN - SCOPUS:67849133172

SN - 0014-5793

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EP - 2468

JO - FEBS Letters

JF - FEBS Letters

IS - 15

ER -

αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets

Abstract

Keywords

ASJC Scopus subject areas

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