Enhancement of Vitamin E Level in Oilseeds through Metabolic Engineering.

(3)The sesame γ-TMT cDNA was ligated to each of the 5'-upstream regions of Δ9 desaturase, oleosin and 2S albumin genes isolated from sesame, or to that of G1 glycinin gene derived from soybean. Such chimera genes were respectively introduced to seedlings of a wild sesame, S.schrxizianum by infection of Agrobacterium with the MAT vector system. For soybean G1 glycinin :: γ-TMT, all the transgenic plants produced immature seeds which seldom germinated. For sesame oleosin :: γ-TMT and sesame 2S albumin :: γ-TMT, the transgenic plants are now during vegetative growth, and for sesameΔ9 desaturase :: γ-TMT, efficient formation of crown galls are observed on the seedlings at present.