Elucidation of regulation mechanisms of RP105/MD-1 in obesity-induced chronic inflammation

The RP105/MD-1 complex expressing cells in hematopoietic cells were involved in high-fat diet-induced adipose tissue inflammation. We found that levels of soluble MD-1 in mice serum were increased by high-fat diet. Soluble MD-1 was dramatically increased by high-fat diet in visceral adipose tissue and produced from stromal vascular cells in the tissue. Soluble MD-1 was increased in co-culture of macrophages and adipocytes, suggesting that macrophages produce soluble MD-1 by the interaction with adipocytes. We identified several candidate lipids from lipid library, which interact with MD-1.