Development and analysis of human monoclonal antibodies derived from patients with cancer

It has been known that the elevation of autoantibodies can frequently be observed in patients with cancer along with its progression. Among those some are potentially useful for the development of therapeutic or diagnostic antibodies for cancer. Recent development of our high-throughput isolation system of human monoclonal antibodies enabled us for the construction of antibody libraries from cancer patients derived antibody-producing single cells. The binding specificity of each antibody was examined by staining the cancer tissue from the same patient. After extensive screening we have succeeded in isolation of multiple antibodies that recognize cancer cells.