In this study, to contribute the consideration of safety measures in maintenance work and fuel control in a fusion reactor, the advanced beta-ray-induced X-ray spectrometry (Advanced-BIXS), which is able to evaluate nondestructively tritium retention in a material in vacuum, was established and its applicability has been investigated. Purpose of the present study was to establish the Advanced-BIXS and basic database on trapping and release behavior of tritium by using it. The applicability and availability of the Advanced-BIXS was examined by measuring tritium-containing samples without air exposure during a cycle of vacuum heating, tritium exposure and measurements of an X-ray spectrum emitted from a sample.