Detection of gastrointestinal cancer biomarkers by next-generation mass spectrometry and comprehensive gene expression analysis

  • 嶋田, 裕 (Principal Investigator)
  • 塚田, 一博 (Co-Investigator(Kenkyū-buntansha))
  • 奥村, 知之 (Co-Investigator(Kenkyū-buntansha))
  • 有馬, 美和子 (Co-Investigator(Kenkyū-buntansha))
  • 安部, 哲也 (Co-Investigator(Kenkyū-buntansha))
  • 門馬, 久美子 (Co-Investigator(Kenkyū-buntansha))
  • 辻本, 豪三 (Co-Investigator(Renkei-kenkyūsha))

Project Details

Abstract

We here evaluated a next-generation mass spectrometer and found that it not suitable for a comprehensive protein analysis of blood. Therefore, we performed a microRNA analysis of blood. To exclude individual differences between patients, we focused on esophageal mucosal cancer patients who were scheduled to undergo endoscopic mucosal resection (EMR) in the present study. Forty-three patients were recruited from 3 institutes. We analyzed the expression of miRNAs in pre-treatment as well as post-treatment blood samples that were collected from 31 patients. Significant differences were observed in the expression of 11 miRNAs between pre-treatment and post-treatment samples. We will analyze these miRNAs in 30 patients with advanced esophageal cancer in a future study and evaluate their efficacy as biomarkers of esophageal cancer.
StatusFinished
Effective start/end date2011/04/01 → 2014/03/31

Funding

  • Japan Society for the Promotion of Science: ¥18,720,000.00

Keywords

  • バイオマーカー
  • 食道癌
  • miRNA
  • 早期発見
  • 質量分析
  • 次世代質量顕微鏡

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