Analysis of proteins regulating bacterial cell division site

We have studied on the Min proteins (MinC,D,E) which regulates position of ceptum ring (FtsZ ring) formation in E.coli. he results of FRET analysis indicated that MinC interacts with FtsZ monomer rather than FtsZ polymer in the solution. It is suggested that interaction between monomeric FtsZ and MinC is important for controlling of FtsZ polymerization by MinC. On the other hand, in vitro analysis of mutant proteins of MinD indicated membrane binding of MinD requires not only ATP binding to nucleotide binding pocket of MinD, but also other factors.