Challenge the infectious diseases and cancers using innovative array technology: Development of hTEC10

We developed a rapid and efficient antibody gene cloning system from a human single B lymphocyte. In this study, by applying the system to T cell receptor, we have developed a rapid and efficient T cell receptor cloning system (hTEC10: human T cell receptor efficient cloning within 10 days),in which we are able to detect an antigen-specific T cells, to clone efficiently TCR-alpha and TCR-beta chain from a single T cell, and finally evaluate the function of T cell receptor by examining reactivity of specialized T cell line (TCR negative cell line). All of the process can be done within 10 days. Using this hTEC10 system, we have been successful in cloning the functional TCRs with high killer activity from peripheral blood lymphocytes of patients with hepatocyte cell carcinoma. This system may contribute TCR gene therapy of infectious diseases as well as various cancers.