TY - JOUR
T1 - Ultrasound enhances liposome-mediated gene transfection
AU - Feril, Loreto B.
AU - Ogawa, Ryohei
AU - Kobayashi, Hideo
AU - Kikuchi, Hiroshi
AU - Kondo, Takashi
N1 - Funding Information:
This study is supported in part by the Grant in Aid for Scientific Research on Priority Areas ©(12217049) from the Ministry of Education, Culture, Sports, Sciences and Technology, and also in part by the 21st Century COE Program of the Toyama Medical and Pharmaceutical University, Japan. We also wish to acknowledge helpful discussions with Dr. P. Riesz, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Maryland, USA.
PY - 2005/8
Y1 - 2005/8
N2 - Previous studies have shown that some series of liposomes, usually containing cationic lipids, are useful tools for gene introduction into cells. To investigate the effect of ultrasound (US) on liposome-mediated transfection, three types of liposomes (designated L1, L2 and L3, in the order of increasing transfection efficiency) containing O,O′-ditetradecanoyl-N-(α- trimethylammonioacetyl) diethanolamine chloride, dioleoylphosphatidylethanolamine, and/or cholesterol at varying ratios, were used in this study. HeLa cells were treated with liposome-DNA complexes containing luciferase genes for 2 h before sonication. Optimal US condition for the enhancement was determined to be 0.5 W/cm2, 1 MHz continuous wave for 1 min and was above threshold for inertial cavitation based on EPR detection of free radicals. Luciferase expressions 24 h after the treatments were significantly increased by sonication to 2.4 fold with L1, and 1.7 fold with L2. However, with L3, which showed the highest level of expression among the liposomes, significant but minimal enhancement was observed when sonication was done 15 min after the DNA-L3 treatment, suggesting that efficiency of the liposome also determines the proper timing for sonication. The 2 h pre-sonication incubation with liposome-DNA complexes for L1 and L2 (30 min for L3) required to attain enhancement, suggests that US works to enhance transfection only after cells had enough DNA uptake.
AB - Previous studies have shown that some series of liposomes, usually containing cationic lipids, are useful tools for gene introduction into cells. To investigate the effect of ultrasound (US) on liposome-mediated transfection, three types of liposomes (designated L1, L2 and L3, in the order of increasing transfection efficiency) containing O,O′-ditetradecanoyl-N-(α- trimethylammonioacetyl) diethanolamine chloride, dioleoylphosphatidylethanolamine, and/or cholesterol at varying ratios, were used in this study. HeLa cells were treated with liposome-DNA complexes containing luciferase genes for 2 h before sonication. Optimal US condition for the enhancement was determined to be 0.5 W/cm2, 1 MHz continuous wave for 1 min and was above threshold for inertial cavitation based on EPR detection of free radicals. Luciferase expressions 24 h after the treatments were significantly increased by sonication to 2.4 fold with L1, and 1.7 fold with L2. However, with L3, which showed the highest level of expression among the liposomes, significant but minimal enhancement was observed when sonication was done 15 min after the DNA-L3 treatment, suggesting that efficiency of the liposome also determines the proper timing for sonication. The 2 h pre-sonication incubation with liposome-DNA complexes for L1 and L2 (30 min for L3) required to attain enhancement, suggests that US works to enhance transfection only after cells had enough DNA uptake.
UR - http://www.scopus.com/inward/record.url?scp=17444415670&partnerID=8YFLogxK
U2 - 10.1016/j.ultsonch.2004.06.006
DO - 10.1016/j.ultsonch.2004.06.006
M3 - 学術論文
C2 - 15848113
AN - SCOPUS:17444415670
SN - 1350-4177
VL - 12
SP - 489
EP - 493
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
IS - 6
ER -