Twisted cell flow facilitates three-dimensional somite morphogenesis in zebrafish

Harunobu Kametani, Yue Tong, Atsuko Shimada, Hiroyuki Takeda*, Takamichi Sushida*, Masakazu Akiyama, Toru Kawanishi

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

抄録

Tissue elongation is a fundamental morphogenetic process to construct complex embryonic structures. In zebrafish, somites rapidly elongate in both dorsal and ventral directions, transforming from a cuboidal to a V-shape within a few hours of development. Despite its significance, the cellular behaviors that directly lead to somite elongation have not been examined at single-cell resolution. Here, we describe the motion and shapes of all cells composing the dorsal half of the somite in three-dimensional space using lightsheet microscopy. We identified two types of cell movements—in horizontal and dorsal directions—that occur simultaneously within individual cells, creating a complex, twisted flow of cells during somite elongation. Chemical inhibition of Sdf1 signaling disrupted the collective movement in both directions and inhibited somite elongation, suggesting that Sdf1 signaling is crucial for this cell flow. Furthermore, three-dimensional computational modeling suggested that horizontal cell rotation accelerates the perpendicular elongation of the somite along the dorsoventral axis. Together, our study offers novel insights into the role of collective cell migration in tissue morphogenesis, which proceeds dynamically in the three-dimensional space of the embryo.

本文言語英語
論文番号203969
ジャーナルCells and Development
180
DOI
出版ステータス出版済み - 2024/12

ASJC Scopus 主題領域

  • 発生生物学

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