TY - JOUR
T1 - Twisted cell flow facilitates three-dimensional somite morphogenesis in zebrafish
AU - Kametani, Harunobu
AU - Tong, Yue
AU - Shimada, Atsuko
AU - Takeda, Hiroyuki
AU - Sushida, Takamichi
AU - Akiyama, Masakazu
AU - Kawanishi, Toru
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/12
Y1 - 2024/12
N2 - Tissue elongation is a fundamental morphogenetic process to construct complex embryonic structures. In zebrafish, somites rapidly elongate in both dorsal and ventral directions, transforming from a cuboidal to a V-shape within a few hours of development. Despite its significance, the cellular behaviors that directly lead to somite elongation have not been examined at single-cell resolution. Here, we describe the motion and shapes of all cells composing the dorsal half of the somite in three-dimensional space using lightsheet microscopy. We identified two types of cell movements—in horizontal and dorsal directions—that occur simultaneously within individual cells, creating a complex, twisted flow of cells during somite elongation. Chemical inhibition of Sdf1 signaling disrupted the collective movement in both directions and inhibited somite elongation, suggesting that Sdf1 signaling is crucial for this cell flow. Furthermore, three-dimensional computational modeling suggested that horizontal cell rotation accelerates the perpendicular elongation of the somite along the dorsoventral axis. Together, our study offers novel insights into the role of collective cell migration in tissue morphogenesis, which proceeds dynamically in the three-dimensional space of the embryo.
AB - Tissue elongation is a fundamental morphogenetic process to construct complex embryonic structures. In zebrafish, somites rapidly elongate in both dorsal and ventral directions, transforming from a cuboidal to a V-shape within a few hours of development. Despite its significance, the cellular behaviors that directly lead to somite elongation have not been examined at single-cell resolution. Here, we describe the motion and shapes of all cells composing the dorsal half of the somite in three-dimensional space using lightsheet microscopy. We identified two types of cell movements—in horizontal and dorsal directions—that occur simultaneously within individual cells, creating a complex, twisted flow of cells during somite elongation. Chemical inhibition of Sdf1 signaling disrupted the collective movement in both directions and inhibited somite elongation, suggesting that Sdf1 signaling is crucial for this cell flow. Furthermore, three-dimensional computational modeling suggested that horizontal cell rotation accelerates the perpendicular elongation of the somite along the dorsoventral axis. Together, our study offers novel insights into the role of collective cell migration in tissue morphogenesis, which proceeds dynamically in the three-dimensional space of the embryo.
KW - Collective cell migration
KW - Sdf1 signaling
KW - Somite elongation
KW - Whole-somite rotation
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85202864799&partnerID=8YFLogxK
U2 - 10.1016/j.cdev.2024.203969
DO - 10.1016/j.cdev.2024.203969
M3 - 学術論文
C2 - 39191372
AN - SCOPUS:85202864799
SN - 2667-291X
VL - 180
JO - Cells and Development
JF - Cells and Development
M1 - 203969
ER -