TY - JOUR
T1 - The presence of Ca2+-independent phospholipase A1 highly specific for phosphatidylinositol in bovine brain
AU - Ueda, Hiroshi
AU - Kobayashi, Tetsuyuki
AU - Kishimoto, Masaaki
AU - Tsutsumi, Tomonari
AU - Watanabe, Shiro
AU - Okuyama, Harumi
PY - 1993/9/30
Y1 - 1993/9/30
N2 - EDTA-insensitive phospholipase A activity hydrolyzing phosphatidylinositol was detected in a bovine brain soluble fraction. This phospholipase A was purified 25-fold by sequential chromatographies of DEAE-Toyopearl, Phenyl-Toyopearl, and Ultrahydrogel 1000. The partially purified EDTA-insensitive phospholipase A showed an apparent molecular mass of 230kDa on an Ultrahydrogel 1000 column in the presence of 0.05% Triton X-100 and a pH optimum at 7.0. The enzyme was highly specific for phosphatidylinositol; phosphatidylethanolamine and phosphatidylcholine were not hydrolyzed significantly. The enzyme activity was characterized as phospholipase A1, and Ca2+ and Mg2+ were not required for its activity. These results indicate the existence of Ca2+-independent, phosphatidylinositol-specific metabolism besides those catalyzed by Ca2+-dependent phospholipase A2 and Ca2+-dependent, phosphatidylinositol-specific phospholipase C.
AB - EDTA-insensitive phospholipase A activity hydrolyzing phosphatidylinositol was detected in a bovine brain soluble fraction. This phospholipase A was purified 25-fold by sequential chromatographies of DEAE-Toyopearl, Phenyl-Toyopearl, and Ultrahydrogel 1000. The partially purified EDTA-insensitive phospholipase A showed an apparent molecular mass of 230kDa on an Ultrahydrogel 1000 column in the presence of 0.05% Triton X-100 and a pH optimum at 7.0. The enzyme was highly specific for phosphatidylinositol; phosphatidylethanolamine and phosphatidylcholine were not hydrolyzed significantly. The enzyme activity was characterized as phospholipase A1, and Ca2+ and Mg2+ were not required for its activity. These results indicate the existence of Ca2+-independent, phosphatidylinositol-specific metabolism besides those catalyzed by Ca2+-dependent phospholipase A2 and Ca2+-dependent, phosphatidylinositol-specific phospholipase C.
UR - http://www.scopus.com/inward/record.url?scp=0027440719&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2181
DO - 10.1006/bbrc.1993.2181
M3 - 学術論文
C2 - 8216258
AN - SCOPUS:0027440719
SN - 0006-291X
VL - 195
SP - 1272
EP - 1279
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -