The development of a gene vector electrostatically assembled with a polysaccharide capsule

Tomoaki Kurosaki, Takashi Kitahara, Shigeru Kawakami, Koyo Nishida, Junzo Nakamura, Mugen Teshima, Hiroo Nakagawa, Yukinobu Kodama, Hideto To, Hitoshi Sasaki*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

75 被引用数 (Scopus)

抄録

The purpose of this study was to develop a gene vector electrostatically assembled with a polysaccharide capsule. We used pDNA/polyethylenimine (PEI) complexes as efficient non-viral vectors. The pDNA/PEI complex was electrostatically encapsulated with various polysaccharides such as fucoidan, λ-carrageenan, xanthan gum, alginic acid, hyaluronic acid, and chondroitin sulfate (CS). The pDNA/PEI complex was shown as nanoparticles with positive ζ-potential, although the ternary complexes encapsulated with polysaccharides were shown as nanoparticles with negative ζ-potential. The pDNA/PEI complex showed high agglutination activity and cytotoxicity, although the ternary complexes encapsulated with polysaccharides had no agglutination activities and lower cytotoxicities. The pDNA/PEI complex showed high uptake and high transgene efficiency in B16-F10 cells. On the other hand, most of the ternary complexes show little uptake and gene expression. The ternary complex encapsulated by CS, however, showed comparable transgene efficiency to the pDNA/PEI complex. The uptake and gene expression of the ternary complex encapsulated by CS were significantly inhibited by hypothermia and the addition of CS, suggesting that the ternary complex was taken by CS-specific receptor-mediated energy-dependent process.

本文言語英語
ページ(範囲)4427-4434
ページ数8
ジャーナルBiomaterials
30
26
DOI
出版ステータス出版済み - 2009/09

ASJC Scopus 主題領域

  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生物理学
  • 生体材料
  • 材料力学

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