The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells

Seiichi Koike*, Kazuko Keino-Masu, Yoko Tanimoto, Satoru Takahashi, Masayuki Masu*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

抄録

Autotaxin, encoded by the Enpp2 gene, is an exoenzyme that produces lysophosphatidic acid, thereby regulating many biologic functions. We previously reported that Enpp2 mRNA was abundantly expressed in yolk sac visceral endoderm (VE) cells and that Enpp2−/− mice were lethal at embryonic day 9.5 owing to angiogenic defects in the yolk sac. Enpp2−/− mice showed lysosome fragmentation in VE cells and embryonic abnormalities including allantois malformation, neural tube defects, no axial turning, and head cavity formation. However, whether the defects in endocytic vesicle formation affect membrane trafficking in VE cells remained to be directly examined. In this study, we found that pinocytosis, transcytosis, and secretion of angiogenic factors such as vascular endothelial growth factor and transforming growth factor β1 were impaired in Enpp2−/− VE cells. Moreover, pharmacologic inhibition of membrane trafficking phenocopied the defects of Enpp2−/− mice. These findings demonstrate that Enpp2 promotes endocytosis and secretion of angiogenic factors in VE cells, thereby regulating angiogenesis/vasculogenesis and embryonic development.

本文言語英語
論文番号bio060081
ジャーナルBiology Open
12
11
DOI
出版ステータス出版済み - 2023/11

ASJC Scopus 主題領域

  • 生化学、遺伝学、分子生物学一般
  • 農業および生物科学一般

フィンガープリント

「The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル